Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607002049_cqe8 GCTGGGATCCTGAGAAGCATCTGTAATCCCAG(+ATATAT)GTACCGAAGGGTTGACACAGGAGGGTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240607002047_5qdr CTTCGGTACCTGGGATTACAGATGCTTCTCAG(+ATATAT)GATCCCAGCCATTCTGCTTTCATTATTAAAG NGG 10 8 17 8 24 0 100 5 10
    240607002045_vkr0 GTAACCTTTAATAATGAAAGCAGAATGGCTGG(+ATATAT)GATCCTGAGAAGCATCTGTAATCCCAGGTAC NGG 10 8 17 8 24 0 100 5 10
    240607002043_g15r TGAACCTGAGACCCTCCTGTGTCAACCCTTAG(+ATATAT)GTACCTGGGATTGCAGATGCTTCTCACTATC NGG 10 8 17 8 24 0 100 5 10
    240607002041_7cmz GCTGGGATAGTGAGAAGCATCTGCAATCCCAG(+ATATAT)GTACCTAAGGGTTGACACAGGAGGGTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240607002039_jmeg GAGCATGCAGCTAAGCAGATATGAGCAGGAGG(+ATATAT)GATCCCATGTAAGAAAGTAAGACACCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240607002037_s5js AAAGTCCTCTGGTGTCTTACTTTCTTACATGG(+ATATAT)GATCCCTCCTGCTCATATCTGCTTAGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240607002036_o4ev GCTGTCTTCAGACATACCAGAAGAGGACATAG(+ATATAT)GATCCTCATTTTATGTGGTTGGTAGGAATTG NGG 10 8 17 8 24 0 100 5 10
    240607002035_5nrh GAGTTCAATTCCTACCAACCACATAAAATGAG(+ATATAT)GATCCTATGTCCTCTTCTGGTATGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240607002034_ywuc CCTAGCTGGGCTCGACCACTTAGCTGGTGAAG(+ATATAT)GATCCTTCCGAGCCCCCAACCCTACCCCAGA NGG 10 8 17 8 24 0 100 5 10