Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607001940_gfvx TAAGTTTGTTTTTGTTTTCCTAAGTAAAATGG(+ATATAT)GATCCAGGATTAGCTAGGTAGAGTCAGGGGA NGG 10 8 17 8 24 0 100 5 10
    240607001938_zo9k AAGTGGATTTCCGAGATGAGGGTTGGCACTTG(+ATATAT)GTACCCACCTGTCGAGTTCTGTGGGACGTCA NGG 10 8 17 8 24 0 100 5 10
    240607001936_6cua GAGCATGACGTCCCACAGAACTCGACAGGTGG(+ATATAT)GTACCAAGTGCCAACCCTCATCTCGGAAATC NGG 10 8 17 8 24 0 100 5 10
    240607001934_bd46 GCTCTGCATTACAAATGTCCTAATGGTGCCAG(+ATATAT)GTACCTTCTGAAGACTCACAGGAGACATTAC NGG 10 8 17 8 24 0 100 5 10
    240607001933_qzrn CTTGAGTAATGTCTCCTGTGAGTCTTCAGAAG(+ATATAT)GTACCTGGCACCATTAGGACATTTGTAATGC NGG 10 8 17 8 24 0 100 5 10
    240607001932_17l0 AAACAAACAATCTATGATAACTCTGTACAGAG(+ATATAT)GTACCAAACCTTTAAGTATTTGTGATGGGAA NGG 10 8 17 8 24 0 100 5 10
    240607001931_i8ku TCAAATTCCCATCACAAATACTTAAAGGTTTG(+ATATAT)GTACCTCTGTACAGAGTTATCATAGATTGTT NGG 10 8 17 8 24 0 100 5 10
    240607001929_76m7 TAATTCAGGGGCTACAGCTCCAAGCCAAGCAG(+ATATAT)GTACCAGGGCACGATGGATCGAAAGGCAATA NGG 10 8 17 8 24 0 100 5 10
    240607001927_4ljn CCACATATTGCCTTTCGATCCATCGTGCCCTG(+ATATAT)GTACCTGCTTGGCTTGGAGCTGTAGCCCCTG NGG 10 8 17 8 24 0 100 5 10
    240607001925_8tly GGCATGGCCTTTCCTCAGAACTTTCTACCATG(+ATATAT)GATCCAAAGCCTAAGACTAGGCCATCGTAGA NGG 10 8 17 8 24 0 100 5 10