Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607001958_1b9q TGTTTCAGTTAAGCTCAAAGGCCACTCATCAG(+ATATAT)GTACCCAAGTTGCCCAAGAACTCAAGGGAGT NGG 10 8 17 8 24 0 100 5 10
    240607001956_5l72 ATGTGGTGCCTGTTCAAAGTCCTGCATAAGGG(+ATATAT)GATCCCTGTACACTCGAACCGTTATTATTTG NGG 10 8 17 8 24 0 100 5 10
    240607001954_7eoo ATCATCAAATAATAACGGTTCGAGTGTACAGG(+ATATAT)GATCCCCTTATGCAGGACTTTGAACAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240607001953_j75h CTGTGTGTAGCATTGAATGGCTTCAGGGTTTG(+ATATAT)GATCCCGTTGAGTGGGGCAAGGTAGGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240607001951_v24a ACTTGGGCCTCCTACCTTGCCCCACTCAACGG(+ATATAT)GATCCAAACCCTGAAGCCATTCAATGCTACA NGG 10 8 17 8 24 0 100 5 10
    240607001948_4bsg CAGCCAGGCTGGGGGTACATGTAGCACCCCTG(+ATATAT)GATCCCATCTTGTTGCTAGCAGGGTAAGTGA NGG 10 8 17 8 24 0 100 5 10
    240607001946_h6sj AGGGGTCACTTACCCTGCTAGCAACAAGATGG(+ATATAT)GATCCAGGGGTGCTACATGTACCCCCAGCCT NGG 10 8 17 8 24 0 100 5 10
    240607001945_n04l GCCTGCTGTTCATCTGACAAGCAAGAGCAGTG(+ATATAT)GATCCATTTCTAAGGCTGTGTACAGGAAGAC NGG 10 8 17 8 24 0 100 5 10
    240607001943_3p9m CCACTGTCTTCCTGTACACAGCCTTAGAAATG(+ATATAT)GATCCACTGCTCTTGCTTGTCAGATGAACAG NGG 10 8 17 8 24 0 100 5 10
    240607001941_ygsk AGTCCTCCCCTGACTCTACCTAGCTAATCCTG(+ATATAT)GATCCCATTTTACTTAGGAAAACAAAAACAA NGG 10 8 17 8 24 0 100 5 10