Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607002142_6j3q TTATTCAAATTTTGATTATTTTAAAAATAAAG(+ATATAT)GATCCATTCTTACCGAAAGGGACACTACATC NGG 10 8 17 8 24 0 100 5 10
    240607002140_n0za TTAAAGATGTAGTGTCCCTTTCGGTAAGAATG(+ATATAT)GATCCTTTATTTTTAAAATAATCAAAATTTG NGG 10 8 17 8 24 0 100 5 10
    240607002139_e9fy CTCCACTTCCCATCTGACCGCTGATGTGCTGG(+ATATAT)GATCCCAGAGCATACTCCTGAGCCTGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240607002137_1yty GTCATAAAGCCAGGCTCAGGAGTATGCTCTGG(+ATATAT)GATCCCAGCACATCAGCGGTCAGATGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240607002136_5nkr TATGTTAATATGATGAAATAAAATCAGAACTG(+ATATAT)GTACCTATTTGTACGTAAGTGTGAGACGTGG NGG 10 8 17 8 24 0 100 5 10
    240607002135_2gfp AGAAGCCACGTCTCACACTTACGTACAAATAG(+ATATAT)GTACCAGTTCTGATTTTATTTCATCATATTA NGG 10 8 17 8 24 0 100 5 10
    240607002133_qjqz TTCAGTTTCCAGTGACCCACAAAAGGGGCGTG(+ATATAT)GTACCGGAAAAACAAAAAGATGGTGTTCTGG NGG 10 8 17 8 24 0 100 5 10
    240607002132_jrk1 CCCTGCCAGAACACCATCTTTTTGTTTTTCCG(+ATATAT)GTACCACGCCCCTTTTGTGGGTCACTGGAAA NGG 10 8 17 8 24 0 100 5 10
    240607002130_m1uk GTATTTGGCTTAATCTCCCCATTTGATATAAG(+ATATAT)GTACCAGTCATTTTTAGAGGCCAAAAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240607002129_5yi5 GGGTTCCTTTTTTTGGCCTCTAAAAATGACTG(+ATATAT)GTACCTTATATCAAATGGGGAGATTAAGCCA NGG 10 8 17 8 24 0 100 5 10