Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607001314_w2gm GCCACTGGAGGGTTGGGCCTGCCCAACTCAGG(+ATATAT)GATCCCAGCTGGAGACATTCTAGCCTTGGGT NGG 10 8 17 8 24 0 100 5 10
    240607001312_lr4d AATCACATTCTCTGTTCAGCCGTTAATGCTGG(+ATATAT)GATCCATATTTAGTTTTATAAGCTTTTCCCT NGG 10 8 17 8 24 0 100 5 10
    240607001311_tl3p AAAACAGGGAAAAGCTTATAAAACTAAATATG(+ATATAT)GATCCCAGCATTAACGGCTGAACAGAGAATG NGG 10 8 17 8 24 0 100 5 10
    240607001309_qept TAGTCACTTCCCTCACCAGTGCAGGTGGGTGG(+ATATAT)GTACCAACCATGCCTCCTCCCCCCCTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607001308_pdvh CCCCCAAAAAAGGGGGGGAGGAGGCATGGTTG(+ATATAT)GTACCCACCCACCTGCACTGGTGAGGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240607001306_1nry GGGCAAGCCGGTTCTCTCCTTCCACCAGATGG(+ATATAT)GATCCCAGGGGATTGAATTCAGGTTATCTGG NGG 10 8 17 8 24 0 100 5 10
    240607001303_er5i AAATCCCAGATAACCTGAATTCAATCCCCTGG(+ATATAT)GATCCCATCTGGTGGAAGGAGAGAACCGGCT NGG 10 8 17 8 24 0 100 5 10
    240607001302_09jt CCTGTGACCCTGCATCCCATGGGTCCTGTGAG(+ATATAT)GTACCCGAGCCCCCTTTCTAACTCCCACCTC NGG 10 8 17 8 24 0 100 5 10
    240607001300_vm4j TTGGGGAGGTGGGAGTTAGAAAGGGGGCTCGG(+ATATAT)GTACCTCACAGGACCCATGGGATGCAGGGTC NGG 10 8 17 8 24 0 100 5 10
    240607001258_01fr CTTTGTCCACTGCAGTAAATTCCACTGTGTGG(+ATATAT)GATCCCCACAGACTTCTGGACAAGGTGGCTT NGG 10 8 17 8 24 0 100 5 10