Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607001241_sy4v GGTGTGGCACAGCTGGAGAAAACTGGCAAGAG(+ATATAT)GATCCCACCTTACAAAGGAGCAAAGAAAACC NGG 10 8 17 8 24 0 100 5 10
    240607001240_u8s7 TCACTGAGATGTGGGACAGGGAGGGCCAGCAG(+ATATAT)GTACCACAGGAAGAACTAGCCCATATAACTA NGG 10 8 17 8 24 0 100 5 10
    240607001237_vmh2 GGCTTTAGTTATATGGGCTAGTTCTTCCTGTG(+ATATAT)GTACCTGCTGGCCCTCCCTGTCCCACATCTC NGG 10 8 17 8 24 0 100 5 10
    240607001236_z49p GAGCCGCATCCAGTGCTCCACGAATTTAGTGG(+ATATAT)GTACCTAGCACTGGGAGTTGAGATGAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240607001234_4p4p TTTTACTTTCTCATCTCAACTCCCAGTGCTAG(+ATATAT)GTACCCACTAAATTCGTGGAGCACTGGATGC NGG 10 8 17 8 24 0 100 5 10
    240607001233_b2is GGCCGAGGAAGTGTGAAGGAAAAGCTGAAGGG(+ATATAT)GATCCCTGTGGGGGTGGGAGGAAGAAAAGAT NGG 10 8 17 8 24 0 100 5 10
    240607001231_owy7 AGACCATCTTTTCTTCCTCCCACCCCCACAGG(+ATATAT)GATCCCCTTCAGCTTTTCCTTCACACTTCCT NGG 10 8 17 8 24 0 100 5 10
    240607001229_6ehm AATGCTTCTAAAGTCAGAGGGCCTTCTCTGGG(+ATATAT)GATCCCCACAAGACTGCTGTCACCAGCCACA NGG 10 8 17 8 24 0 100 5 10
    240607001226_hgkv TGATCTGTGGCTGGTGACAGCAGTCTTGTGGG(+ATATAT)GATCCCCAGAGAAGGCCCTCTGACTTTAGAA NGG 10 8 17 8 24 0 100 5 10
    240607001225_uva7 AGGCAGCACTCAGGAGGCAGAGACAGGTGGTG(+ATATAT)GATCCGAATTCAAGAGCAGTCTGGGCTACAT NGG 10 8 17 8 24 0 100 5 10