Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607001256_hyg1 TGGGGAAGCCACCTTGTCCAGAAGTCTGTGGG(+ATATAT)GATCCCACACAGTGGAATTTACTGCAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240607001255_32tk TGCCCCAGGCTCCAGAGCAAGTAGGACGTCAG(+ATATAT)GTACCTGCTCTCTTGCCTGGCTCTAGATTTT NGG 10 8 17 8 24 0 100 5 10
    240607001254_csx3 AAATTAAAATCTAGAGCCAGGCAAGAGAGCAG(+ATATAT)GTACCTGACGTCCTACTTGCTCTGGAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240607001252_g0hj GCCAAGTGGGACGCATTGACTGGTCACACGTG(+ATATAT)GTACCTCTGGCCTTTCTTTTATTTATTTATT NGG 10 8 17 8 24 0 100 5 10
    240607001251_8n5z AAATAAATAAATAAATAAAAGAAAGGCCAGAG(+ATATAT)GTACCACGTGTGACCAGTCAATGCGTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240607001249_t0j4 CCCTGGGCCAAACATATTCAAACCACCATAAG(+ATATAT)GTACCCAGAGAACAACCCCAGAACTGCACTA NGG 10 8 17 8 24 0 100 5 10
    240607001248_tycs TCTCATAGTGCAGTTCTGGGGTTGTTCTCTGG(+ATATAT)GTACCTTATGGTGGTTTGAATATGTTTGGCC NGG 10 8 17 8 24 0 100 5 10
    240607001246_vww9 CTTAGTCCTGAGACTGCCAGACAATCAAGCAG(+ATATAT)GTACCCCACCAGGAGGCCAGCTCTGGACCTC NGG 10 8 17 8 24 0 100 5 10
    240607001244_rfnr TGGCAGAGGTCCAGAGCTGGCCTCCTGGTGGG(+ATATAT)GTACCTGCTTGATTGTCTGGCAGTCTCAGGA NGG 10 8 17 8 24 0 100 5 10
    240607001242_ti4d CTTAGGGTTTTCTTTGCTCCTTTGTAAGGTGG(+ATATAT)GATCCTCTTGCCAGTTTTCTCCAGCTGTGCC NGG 10 8 17 8 24 0 100 5 10