Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607001152_tqxf GGGGTTGTCGCCGCTTTTACGTGAGCGGCGTG(+ATATAT)GATCCACGCAGCGATGCCGTCTACTTTGAGA NGG 10 8 17 8 24 0 100 5 10
    240607001150_2efc TCCTTTGCCTTATCTCATGGGGATGGAGGAGG(+ATATAT)GTACCCAGGTCTCTGTTGCTTTGACTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240607001148_7kfh TTATTGAGACAGTCAAAGCAACAGAGACCTGG(+ATATAT)GTACCCTCCTCCATCCCCATGAGATAAGGCA NGG 10 8 17 8 24 0 100 5 10
    240607001147_xjv5 AGTACCTGAGGGTCTGTAGGTTGCACACTTTG(+ATATAT)GTACCAGGTAACTTTTTTTTCTTTATAAGAA NGG 10 8 17 8 24 0 100 5 10
    240607001145_6ksy ACTCTTTCTTATAAAGAAAAAAAAGTTACCTG(+ATATAT)GTACCAAAGTGTGCAACCTACAGACCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240607001144_ti66 TCTTCCCACTGTGTAGTCCTGGGTCTTGTTAG(+ATATAT)GATCCTGGCTGGGGCCTCTGGGAGCAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240607001142_7f6s GCTCGACTTTTGCTCCCAGAGGCCCCAGCCAG(+ATATAT)GATCCTAACAAGACCCAGGACTACACAGTGG NGG 10 8 17 8 24 0 100 5 10
    240607001140_37rr AGCAGTCTCCCAGTGCATGTTTGCATAGCTCG(+ATATAT)GTACCAGAGCGGTTTGTAATTGAGCCTGTTT NGG 10 8 17 8 24 0 100 5 10
    240607001139_fnsg ACTGCAAACAGGCTCAATTACAAACCGCTCTG(+ATATAT)GTACCGAGCTATGCAAACATGCACTGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240607001138_95kd GCTCTTTAAGGCAGAGGAGACAGACATGTGTG(+ATATAT)GATCCGGGGCTGTTGGCTCATCTCACGTGGT NGG 10 8 17 8 24 0 100 5 10