Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607001341_x2qf GTGGACTGCATCCGACTGAAATAAGCAAAATG(+ATATAT)GTACCACAGCGCCCGGGTGCACACAGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240607001339_5wor TTCATGGCTGCTGTGTGCACCCGGGCGCTGTG(+ATATAT)GTACCATTTTGCTTATTTCAGTCGGATGCAG NGG 10 8 17 8 24 0 100 5 10
    240607001337_w2e6 GTAAGACTGGGAGAAAGATTTCTTAACCAGTG(+ATATAT)GTACCTGACTCTTGACCTGGTCATGGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240607001336_60vv ATTCCCAGGACCATGACCAGGTCAAGAGTCAG(+ATATAT)GTACCACTGGTTAAGAAATCTTTCTCCCAGT NGG 10 8 17 8 24 0 100 5 10
    240607001335_tras AGAATTTTCACGGCAGGTACATAAAGGAAGTG(+ATATAT)GTACCCAGTCTCCAGTCAGGACGTGAGCACC NGG 10 8 17 8 24 0 100 5 10
    240607001333_wkik AGCCTGGTGCTCACGTCCTGACTGGAGACTGG(+ATATAT)GTACCACTTCCTTTATGTACCTGCCGTGAAA NGG 10 8 17 8 24 0 100 5 10
    240607001330_krxq TTGGACTGGGAGCCAGGCCATCTCCACCCCTG(+ATATAT)GTACCACCCAGCAACTCTGAAAATGTGCAGT NGG 10 8 17 8 24 0 100 5 10
    240607001328_kwaz GGGACACTGCACATTTTCAGAGTTGCTGGGTG(+ATATAT)GTACCAGGGGTGGAGATGGCCTGGCTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240607001327_8gsr ACTACTCTTAAGAGATGAAGAAATAAGGAGTG(+ATATAT)GATCCCTGTGTCCTATCTTCAAATGTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240607001325_4mw4 ATGTGGCTGGACATTTGAAGATAGGACACAGG(+ATATAT)GATCCACTCCTTATTTCTTCATCTCTTAAGA NGG 10 8 17 8 24 0 100 5 10