Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220147_9q7c TCAGGTATATTGCCCTGTAAATTGTGGTAGTG(+ATATAT)GTACCAGGAATAAAAAATTAAGGAATTTTTA NGG 10 8 17 8 24 0 100 5 10
    240606220145_x1wb CACTCAGGCCTGGAACTCGGTGCCCCCTCTCG(+ATATAT)GATCCTGGCCTCACATGGGCCTCAGACCTAA NGG 10 8 17 8 24 0 100 5 10
    240606220143_jy5x TTTTCTTAGGTCTGAGGCCCATGTGAGGCCAG(+ATATAT)GATCCGAGAGGGGGCACCGAGTTCCAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240606220142_jxsl ACTCTAGGATTGGAAAAAAAAAAAAAAAAAAG(+ATATAT)GTACCGCAGCCTCTTGGCAAATAAGGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606220141_rk1h GTTCCAGAAACCTTATTTGCCAAGAGGCTGCG(+ATATAT)GTACCTTTTTTTTTTTTTTTTTTCCAATCCT NGG 10 8 17 8 24 0 100 5 10
    240606220139_gqkz AGCTCCTTGTCACCATGTTAAGAGATCATTTG(+ATATAT)GATCCTCTAATTAAGTTTAAAATTTGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240606220138_adgu AAGGAAAAAACAAATTTTAAACTTAATTAGAG(+ATATAT)GATCCAAATGATCTCTTAACATGGTGACAAG NGG 10 8 17 8 24 0 100 5 10
    240606220136_c07l GATGTTTACATTTACCATGTTCCCCTTATTAG(+ATATAT)GATCCGCACAGCCAAGTTCCGGGCCTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606220135_czqg CCTGAGGAAGAGGCCCGGAACTTGGCTGTGCG(+ATATAT)GATCCTAATAAGGGGAACATGGTAAATGTAA NGG 10 8 17 8 24 0 100 5 10
    240606220134_x6rc CCACACACACACACACACGGGCCAGTCAGACG(+ATATAT)GATCCAGGTGTCCAGTTGTGGCTGATCAGCC NGG 10 8 17 8 24 0 100 5 10