Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220059_qzeu GTACACTCTTCAGGACCACCTGTCTAGGGCTG(+ATATAT)GTACCAGCCACAAGGGCTGCTTCCTCCCACA NGG 10 8 17 8 24 0 100 5 10
    240606220058_2onh ATTGATGTGGGAGGAAGCAGCCCTTGTGGCTG(+ATATAT)GTACCAGCCCTAGACAGGTGGTCCTGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240606220055_x85m AATCCTTTGAGTGAACCTAGTGTTTCCGGCTG(+ATATAT)GATCCTGAGAGATACAAATGGAAAAATACTG NGG 10 8 17 8 24 0 100 5 10
    240606220054_uc6h CAGGTCAGTATTTTTCCATTTGTATCTCTCAG(+ATATAT)GATCCAGCCGGAAACACTAGGTTCACTCAAA NGG 10 8 17 8 24 0 100 5 10
    240606220053_vxwp AGGATACTTAAGTCATCAAAGACATTGGTATG(+ATATAT)GATCCGCTGTGGTGGTACATGCTGTAATCTC NGG 10 8 17 8 24 0 100 5 10
    240606220051_bqcj GTGCTGAGATTACAGCATGTACCACCACAGCG(+ATATAT)GATCCATACCAATGTCTTTGATGACTTAAGT NGG 10 8 17 8 24 0 100 5 10
    240606220048_0rdb AGCACTGGGCTCGCTTGGCATGTCCTGGGTTG(+ATATAT)GATCCCAGTACTCCAACTGAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606220047_2e7v CTTTTTTTTTTTTTTTTCAGTTGGAGTACTGG(+ATATAT)GATCCAACCCAGGACATGCCAAGCGAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240606220045_jzct GCAAGCTGCTTCCCCCGAGCCTTCAGCACAGG(+ATATAT)GATCCTCTCAACCACAGGCAGCCGGGTTGCC NGG 10 8 17 8 24 0 100 5 10
    240606220044_9fjg TTGCTGGCAACCCGGCTGCCTGTGGTTGAGAG(+ATATAT)GATCCCTGTGCTGAAGGCTCGGGGGAAGCAG NGG 10 8 17 8 24 0 100 5 10