Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220115_aree TGTATTCAGGACCTGAAGTGAGTACCCGTGTG(+ATATAT)GATCCAGACCAATCCAGTGTGAGACTACTGA NGG 10 8 17 8 24 0 100 5 10
    240606220114_ve5f ACGGAGCAGCCGGAAACAAGGAGGCCTCGTGG(+ATATAT)GATCCTTTAGTGCCAAGCCTGGCGTCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606220112_9fdw CAAGTACAGACCGAGGCCCAGGAGAACGGGCG(+ATATAT)GTACCGTGTCCAGTCAGCTGCATGGTACTGT NGG 10 8 17 8 24 0 100 5 10
    240606220110_4a2t CGCTGACAGTACCATGCAGCTGACTGGACACG(+ATATAT)GTACCGCCCGTTCTCCTGGGCCTCGGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240606220109_49yj TGAGCTAATTGTGTGTGTGCCTTTCATCTGAG(+ATATAT)GATCCGAGAGAGCTCCTGGGCAGGTACGAGC NGG 10 8 17 8 24 0 100 5 10
    240606220108_3hm2 CGCAGGCTCGTACCTGCCCAGGAGCTCTCTCG(+ATATAT)GATCCTCAGATGAAAGGCACACACACAATTA NGG 10 8 17 8 24 0 100 5 10
    240606220106_qpxz TACCCTAGTTCTATAGAGCAGCTTGGACACTG(+ATATAT)GTACCTTGGTCTTCTGCCTTGAAGCTGATTT NGG 10 8 17 8 24 0 100 5 10
    240606220104_zfvd CATGGAAATCAGCTTCAAGGCAGAAGACCAAG(+ATATAT)GTACCAGTGTCCAAGCTGCTCTATAGAACTA NGG 10 8 17 8 24 0 100 5 10
    240606220102_0z79 GTTAGGATTGCAACATGCCTCACTATCTCAAG(+ATATAT)GTACCTAGATTTTTATTTGCTAAATCTAAAT NGG 10 8 17 8 24 0 100 5 10
    240606220101_o0yz TTGATATTTAGATTTAGCAAATAAAAATCTAG(+ATATAT)GTACCTTGAGATAGTGAGGCATGTTGCAATC NGG 10 8 17 8 24 0 100 5 10