Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220024_drj3 GTCTTCTTCTCTGCCAGATAACAGAGCCATTG(+ATATAT)GATCCACTTATTAAAGACTTCTTATCACTTA NGG 10 8 17 8 24 0 100 5 10
    240606220022_fdyf AATGTTAAGTGATAAGAAGTCTTTAATAAGTG(+ATATAT)GATCCAATGGCTCTGTTATCTGGCAGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606220020_4bo5 CTTTTTCTAGAGTCCTTTCTACACACACTTTG(+ATATAT)GTACCCTGGGGAGTTTGGATGCAACCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606220016_autk CCTCCTTCCTGGTTGCATCCAAACTCCCCAGG(+ATATAT)GTACCAAAGTGTGTGTAGAAAGGACTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240606220014_zoak CAAGACCTTCAAGAAGGTGGGCTCCGGGGATG(+ATATAT)GTACCATTGCTCTTTCCTAGCTAACCCTAGA NGG 10 8 17 8 24 0 100 5 10
    240606220013_3ng5 CCGTATCTAGGGTTAGCTAGGAAAGAGCAATG(+ATATAT)GTACCATCCCCGGAGCCCACCTTCTTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240606220011_083p AAATGGTCCATATAAATTTGTGGCATCAATGG(+ATATAT)GATCCCAACTTGGCTTCCCTAGTCTTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240606220009_03pq CGTGGGAAGGAAGACTAGGGAAGCCAAGTTGG(+ATATAT)GATCCCATTGATGCCACAAATTTATATGGAC NGG 10 8 17 8 24 0 100 5 10
    240606220007_y0dp AGGACCATGACAAAGCCCCTGAGATCAAGGTG(+ATATAT)GTACCAGTTGCAGCAGCAATGTGGTGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606220005_q24v TAAGGCTGAGCACCACATTGCTGCTGCAACTG(+ATATAT)GTACCACCTTGATCTCAGGGGCTTTGTCATG NGG 10 8 17 8 24 0 100 5 10