Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220132_nd25 CCCGAGGCTGATCAGCCACAACTGGACACCTG(+ATATAT)GATCCGTCTGACTGGCCCGTGTGTGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606220131_kzlk TTAGGGCCCAAGAGACGGCCCAGCAGGTAAAG(+ATATAT)GTACCTGCTGCCAGGTCTAATGAGCTAAGTT NGG 10 8 17 8 24 0 100 5 10
    240606220129_wbnv AATCAAACTTAGCTCATTAGACCTGGCAGCAG(+ATATAT)GTACCTTTACCTGCTGGGCCGTCTCTTGGGC NGG 10 8 17 8 24 0 100 5 10
    240606220128_wbkp ACATGTGGTTGCCATGGCTTAATGTATGGACG(+ATATAT)GATCCTCCACTGTCTTGCAATTCATTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240606220126_69g8 TTTTTCAACAAATGAATTGCAAGACAGTGGAG(+ATATAT)GATCCGTCCATACATTAAGCCATGGCAACCA NGG 10 8 17 8 24 0 100 5 10
    240606220124_em1d CACCTTTTCTACAGCAATTTTTAGTAATTATG(+ATATAT)GTACCAAGTTCCCACAAACCTTTGGGTGACT NGG 10 8 17 8 24 0 100 5 10
    240606220123_7cm6 TACCCAGTCACCCAAAGGTTTGTGGGAACTTG(+ATATAT)GTACCATAATTACTAAAAATTGCTGTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606220121_xvng AGCCTCACCTGGTCCCTGGCCTTCAGCCTCTG(+ATATAT)GATCCCTGGGTGGGACCCAGAGAATCTCACG NGG 10 8 17 8 24 0 100 5 10
    240606220118_h57x ATGAGCGTGAGATTCTCTGGGTCCCACCCAGG(+ATATAT)GATCCAGAGGCTGAAGGCCAGGGACCAGGTG NGG 10 8 17 8 24 0 100 5 10
    240606220117_sdqr GTTCTTCAGTAGTCTCACACTGGATTGGTCTG(+ATATAT)GATCCACACGGGTACTCACTTCAGGTCCTGA NGG 10 8 17 8 24 0 100 5 10