Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606220002_z43f TGATGTGACTAGAAAGGCCTGGCCACTTTAGG(+ATATAT)GTACCAAGGGAGAACTCCAAGGAGCATTTTT NGG 10 8 17 8 24 0 100 5 10
    240606215959_ztkw GGGGGAAAAATGCTCCTTGGAGTTCTCCCTTG(+ATATAT)GTACCCTAAAGTGGCCAGGCCTTTCTAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606215957_rsdc ATTGCCTTATGTAGTAATCAGCAACTGAAGGG(+ATATAT)GTACCCAACCTCTACAGTCTTCCAGCACAAT NGG 10 8 17 8 24 0 100 5 10
    240606215954_46lh GGGCAATTGTGCTGGAAGACTGTAGAGGTTGG(+ATATAT)GTACCCCTTCAGTTGCTGATTACTACATAAG NGG 10 8 17 8 24 0 100 5 10
    240606215952_gqvz TCAAAGCCTTCTGAGACCCCATCCCACACGAG(+ATATAT)GTACCGTGAAGGGGCACTAAAGTGCATTCTG NGG 10 8 17 8 24 0 100 5 10
    240606215950_cetg TCCTTCAGAATGCACTTTAGTGCCCCTTCACG(+ATATAT)GTACCTCGTGTGGGATGGGGTCTCAGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240606215948_k5hb CTTGAGCATCATACTGCTTTTAAATCCATGGG(+ATATAT)GATCCTGGAAGAGCTCCCCTACAGATACTGA NGG 10 8 17 8 24 0 100 5 10
    240606215945_f8hl GCCCCTCAGTATCTGTAGGGGAGCTCTTCCAG(+ATATAT)GATCCCCATGGATTTAAAAGCAGTATGATGC NGG 10 8 17 8 24 0 100 5 10
    240606215943_nloo AATAAAGCTGGACTGAAAGAAACATCTCTGAG(+ATATAT)GATCCAGGAAGTGGACTGTCATTCACCAAAT NGG 10 8 17 8 24 0 100 5 10
    240606215941_xib6 AGTTGATTTGGTGAATGACAGTCCACTTCCTG(+ATATAT)GATCCTCAGAGATGTTTCTTTCAGTCCAGCT NGG 10 8 17 8 24 0 100 5 10