Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606182548_tv27 TAATAAGCAGAAAATAGCATACCCTGATTGTG(+AAAAAA)GTACCTTTGAAGGCTGGGGGTGGGGGGTGCT NGG 10 8 17 8 24 0 100 5 10
    240606182546_jed0 GAACCTTACAAGTAGAGATTGATAAAGAGAAG(+AAAAAA)GTACCCTACCTCCTCTCTCTCTCTCGCTCTC NGG 10 8 17 8 24 0 100 5 10
    240606182544_06ej AGAGAGAGAGCGAGAGAGAGAGAGGAGGTAGG(+AAAAAA)GTACCTTCTCTTTATCAATCTCTACTTGTAA NGG 10 8 17 8 24 0 100 5 10
    240606182541_33ob CCCTCAACTGCCTATTAATTAAAACTCCAGGG(+AAAAAA)GATCCAATGTTCTCTTCTGACTTCTGCATTC NGG 10 8 17 8 24 0 100 5 10
    240606182540_0ats CATGTGAATGCAGAAGTCAGAAGAGAACATTG(+AAAAAA)GATCCCCTGGAGTTTTAATTAATAGGCAGTT NGG 10 8 17 8 24 0 100 5 10
    240606182539_lud0 GAAAAGGAGGTTCAGCGACAGACCCAAAGTAG(+AAAAAA)GATCCAGATCAAGGAGATGTCCCAAGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240606182538_kjgy AGTGTGAGGCCTTGGGACATCTCCTTGATCTG(+AAAAAA)GATCCTACTTTGGGTCTGTCGCTGAACCTCC NGG 10 8 17 8 24 0 100 5 10
    240606182535_1c6t TCCTCAGAAAATTGGACATAGTACTACCGGAG(+AAAAAA)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240606182534_hcun TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+AAAAAA)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606182532_zfh1 GGATGGACCATGTAGAGACTGCCATATCCAGG(+AAAAAA)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10