Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606182441_gcyt GCCTTCTTCGTTGTCAACTCGGGGCACTCCTG(+AAAAAA)GATCCTATGAGCCCCGGACAAGGTGCATTGT NGG 10 8 17 8 24 0 100 5 10
    240606182440_6g0a TTAGGGGAGAGAGTAAGGAGGATACTGATTAG(+AAAAAA)GTACCAAGGTTTCATTACCTTAAAGGCAAAA NGG 10 8 17 8 24 0 100 5 10
    240606182438_5lmm CTGGTTTTTGCCTTTAAGGTAATGAAACCTTG(+AAAAAA)GTACCTAATCAGTATCCTCCTTACTCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240606182437_39qk ATGGGACCTATTCCAGCCCCTTGAATCTTAAG(+AAAAAA)GTACCATATGAGAGCTAAGAGCAGGTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606182436_hbuy ATGCTGGGAAACCTGCTCTTAGCTCTCATATG(+AAAAAA)GTACCTTAAGATTCAAGGGGCTGGAATAGGT NGG 10 8 17 8 24 0 100 5 10
    240606182434_saez CTTGCTCTCCAGGAGCTCACAGTTCAAGCTGG(+AAAAAA)GATCCAGATGAGCAAACATGATCATCACACA NGG 10 8 17 8 24 0 100 5 10
    240606182433_rxid TATACTGTGTGATGATCATGTTTGCTCATCTG(+AAAAAA)GATCCCAGCTTGAACTGTGAGCTCCTGGAGA NGG 10 8 17 8 24 0 100 5 10
    240606182431_py4d GGTGTCAGAGTTTGGATGCTGATTATGGGGTG(+AAAAAA)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606182429_jqjk GGATGGACCATGTAGAGACTGCCATATCCAGG(+AAAAAA)GATCCACCCCATAATCAGCATCCAAACTCTG NGG 10 8 17 8 24 0 100 5 10
    240606182428_jr2p ACGGACTGCTAAATTGGGTCCTATAGATCGAG(+AAAAAA)GTACCATAATTAAGATATTTTAATAAGGCTC NGG 10 8 17 8 24 0 100 5 10