Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606182340_crfm AAACATATTGAAAAGAATATAAAAAAGCAGTG(+AAAAAA)GATCCATCACATATAATTTTGTGGCCATAAT NGG 10 8 17 8 24 0 100 5 10
    240606182338_rogw TATGAAACATTGAAACAACAGGCCCAGGAGGG(+AAAAAA)GATCCAGCCTCTAATCCAGGTTTGATTTGCA NGG 10 8 17 8 24 0 100 5 10
    240606182337_hsdr ACAAATGCAAATCAAACCTGGATTAGAGGCTG(+AAAAAA)GATCCCCTCCTGGGCCTGTTGTTTCAATGTT NGG 10 8 17 8 24 0 100 5 10
    240606182336_pfi5 CTCAGTATGTATTTGACTGTATGAAATAAATG(+AAAAAA)GATCCTTCTATGTGTCTCTATATATGTCTAT NGG 10 8 17 8 24 0 100 5 10
    240606182334_pahi AGAGAATAGACATATATAGAGACACATAGAAG(+AAAAAA)GATCCATTTATTTCATACAGTCAAATACATA NGG 10 8 17 8 24 0 100 5 10
    240606182332_fk3j CTGCAAAGGGAAGGAGTACAGGAAGCGCCATG(+AAAAAA)GATCCTACAGTGTTAGTTTGTCAGCTAGCCA NGG 10 8 17 8 24 0 100 5 10
    240606182331_o9ed CATGTTGGCTAGCTGACAAACTAACACTGTAG(+AAAAAA)GATCCATGGCGCTTCCTGTACTCCTTCCCTT NGG 10 8 17 8 24 0 100 5 10
    240606182330_r6vy CAATACAGTAAGTTCAATTGAGAGGCCAAACG(+AAAAAA)GATCCAAGGAAGATAAAAATTAAATACTTAA NGG 10 8 17 8 24 0 100 5 10
    240606182328_srab TTAATTTAAGTATTTAATTTTTATCTTCCTTG(+AAAAAA)GATCCGTTTGGCCTCTCAATTGAACTTACTG NGG 10 8 17 8 24 0 100 5 10
    240606182326_8gl1 CTGGGTCTTCCCAGAGCAGACATTTTCACCAG(+AAAAAA)GTACCTTACCCAAAACAAGTGAGGTGATCTC NGG 10 8 17 8 24 0 100 5 10