Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606182325_ukan CCTCTGAGATCACCTCACTTGTTTTGGGTAAG(+AAAAAA)GTACCTGGTGAAAATGTCTGCTCTGGGAAGA NGG 10 8 17 8 24 0 100 5 10
    240606182324_7j25 AATCCAAATCTTCATTCATTTCTGGCACAAAG(+AAAAAA)GTACCAGGAAATGATGTACTATCAGGAAATC NGG 10 8 17 8 24 0 100 5 10
    240606182322_uq8z TGTGTGATTTCCTGATAGTACATCATTTCCTG(+AAAAAA)GTACCTTTGTGCCAGAAATGAATGAAGATTT NGG 10 8 17 8 24 0 100 5 10
    240606182320_1yu0 AGATTTAAAAACACCAAAAGCTGCTATCCTTG(+AAAAAA)GTACCAAGAACTGTGCATATGTGTTCAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606182319_s1qz GTCTATTTCTGAACACATATGCACAGTTCTTG(+AAAAAA)GTACCAAGGATAGCAGCTTTTGGTGTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240606182317_nhg9 TCGTTTTGCTGAGCAATCTAAATGCCTCGATG(+AAAAAA)GTACCCAGAATGTGTTACTTCTTTTAGATTT NGG 10 8 17 8 24 0 100 5 10
    240606182316_lhfw TTACAAAATCTAAAAGAAGTAACACATTCTGG(+AAAAAA)GTACCATCGAGGCATTTAGATTGCTCAGCAA NGG 10 8 17 8 24 0 100 5 10
    240606182315_lfl0 GTCCTGCCTACCAACTAGGGCCAAGTCTGCTG(+AAAAAA)GATCCTGCCAGTGGGGTCTTAAGTATAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606182313_3gfz CGGGATCTTTATACTTAAGACCCCACTGGCAG(+AAAAAA)GATCCAGCAGACTTGGCCCTAGTTGGTAGGC NGG 10 8 17 8 24 0 100 5 10
    240606182312_bkty AAATTGTTCTGTGTATTTGTCACATATTTAAG(+AAAAAA)GATCCACGCTTCTTATATTTTCTTTTATTAT NGG 10 8 17 8 24 0 100 5 10