Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606182311_jjbg AACTTATAATAAAAGAAAATATAAGAAGCGTG(+AAAAAA)GATCCTTAAATATGTGACAAATACACAGAAC NGG 10 8 17 8 24 0 100 5 10
    240606182310_li90 GATGTTCATTATTCCTAAATAGCAGCTCAAAG(+AAAAAA)GATCCCTTTGCTACACAAGCGCAATGAAAAC NGG 10 8 17 8 24 0 100 5 10
    240606182308_8g05 CTTAGGTTTTCATTGCGCTTGTGTAGCAAAGG(+AAAAAA)GATCCTTTGAGCTGCTATTTAGGAATAATGA NGG 10 8 17 8 24 0 100 5 10
    240606182307_ibhp AATGTGTGTATTTCTTTATCTCTTACTCTATG(+AAAAAA)GTACCATGAGATCATTCAGCCGTCCTTGTCA NGG 10 8 17 8 24 0 100 5 10
    240606182305_4t1j ACCTTTGACAAGGACGGCTGAATGATCTCATG(+AAAAAA)GTACCATAGAGTAAGAGATAAAGAAATACAC NGG 10 8 17 8 24 0 100 5 10
    240606182304_vw1c CTTGCCTACATATGGAGCAGTGACTGCTACTG(+AAAAAA)GATCCCTAAGCAATAGATTCATTTGGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240606182302_3lw8 TGTAATGCCTCCAAATGAATCTATTGCTTAGG(+AAAAAA)GATCCAGTAGCAGTCACTGCTCCATATGTAG NGG 10 8 17 8 24 0 100 5 10
    240606182301_le3b TTGGATCTAGCACATAATAACAAGGCATAGTG(+AAAAAA)GTACCTGCCTGTTCTTGTGCTGAGACGTGTA NGG 10 8 17 8 24 0 100 5 10
    240606182300_6v4g CTGCATACACGTCTCAGCACAAGAACAGGCAG(+AAAAAA)GTACCACTATGCCTTGTTATTATGTGCTAGA NGG 10 8 17 8 24 0 100 5 10
    240606182258_zehu CCTGTTCTTGTGCTGAGACGTGTATGCAGTGG(+AAAAAA)GATCCTGAGGGCTTTCCTGGACAGTCAATTT NGG 10 8 17 8 24 0 100 5 10