Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606182208_cv4c ATTTATTTGATTTGATCATTTCTTCACATGTG(+AAAAAA)GTACCAAATTCAACAATGAAGCACAACCTCA NGG 10 8 17 8 24 0 100 5 10
    240606182206_au5n ACAACATTTTAGGACTTAAACATTTGATTTCG(+AAAAAA)GTACCAGATGAATTGCTAACCTGTTACTGAT NGG 10 8 17 8 24 0 100 5 10
    240606182205_knox AAATTATCAGTAACAGGTTAGCAATTCATCTG(+AAAAAA)GTACCGAAATCAAATGTTTAAGTCCTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606182203_plob GTCTCGGATTTGCTTGTTTGAGACCTTCTAAG(+AAAAAA)GTACCAGTGCACACTTAGTTGCTTATTTCAC NGG 10 8 17 8 24 0 100 5 10
    240606182202_m0is TAAGAGTGAAATAAGCAACTAAGTGTGCACTG(+AAAAAA)GTACCTTAGAAGGTCTCAAACAAGCAAATCC NGG 10 8 17 8 24 0 100 5 10
    240606182201_o0hk ATTAATATTTAGAGTAGGAAGCAGCCTCTTAG(+AAAAAA)GTACCACAGTTAAAAACTAAGAGAACGAACA NGG 10 8 17 8 24 0 100 5 10
    240606182159_5gpg AATTTTGTTCGTTCTCTTAGTTTTTAACTGTG(+AAAAAA)GTACCTAAGAGGCTGCTTCCTACTCTAAATA NGG 10 8 17 8 24 0 100 5 10
    240606182158_vsdo TGGGAAAGTGTGGGATCACCCCCTGGCCTTTG(+AAAAAA)GATCCTCCCCCATATATACTTTCTAACTCTT NGG 10 8 17 8 24 0 100 5 10
    240606182156_fzuf CCGCAAAGAGTTAGAAAGTATATATGGGGGAG(+AAAAAA)GATCCAAAGGCCAGGGGGTGATCCCACACTT NGG 10 8 17 8 24 0 100 5 10
    240606182155_y1im ATTTAGTGTGTCAAAACGAACCGTACTCAAAG(+AAAAAA)GATCCACTAGTGTCCAATTCAAAAATGGCTG NGG 10 8 17 8 24 0 100 5 10