Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606182122_j4ep TACTCCAGTAACAATTCTAATGCAATTGACTG(+AAAAAA)GATCCCACCAACTGGGAAGATGAAGGGGATT NGG 10 8 17 8 24 0 100 5 10
    240606182120_3sqm TCCCTCCCCACCGACATGACTCCCAAAGTGGG(+AAAAAA)GTACCAGACAGCATGGGGGTTAAGATTAGTT NGG 10 8 17 8 24 0 100 5 10
    240606182119_dua1 TAGGAAACTAATCTTAACCCCCATGCTGTCTG(+AAAAAA)GTACCCCACTTTGGGAGTCATGTCGGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606182116_odso GCTGGTTCCAGCTGCTGTTCCATGGGCCCTTG(+AAAAAA)GTACCAAGGACTCGTATGTGTCCCCATTTGA NGG 10 8 17 8 24 0 100 5 10
    240606182114_099o GCCCTTCAAATGGGGACACATACGAGTCCTTG(+AAAAAA)GTACCAAGGGCCCATGGAACAGCAGCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240606182112_sxyt GGGGTATACTCCAGTTATCACCCAACCTGGGG(+AAAAAA)GTACCCTCCAGTTATCACCCAACCACCAGAC NGG 10 8 17 8 24 0 100 5 10
    240606182110_6755 CATTAGTCTGGTGGTTGGGTGATAACTGGAGG(+AAAAAA)GTACCCCCAGGTTGGGTGATAACTGGAGTAT NGG 10 8 17 8 24 0 100 5 10
    240606182109_isie CGCTAACCTCCCCTACTCACCTTGGCAATCTG(+AAAAAA)GATCCAATGCTCACTGCCTTCCCTTGGGGCT NGG 10 8 17 8 24 0 100 5 10
    240606182106_e3ot AACTTAGCCCCAAGGGAAGGCAGTGAGCATTG(+AAAAAA)GATCCAGATTGCCAAGGTGAGTAGGGGAGGT NGG 10 8 17 8 24 0 100 5 10
    240606182105_n1fk TGAGAGGGATGTGTGTGTGTGTGTGTGTGTAG(+AAAAAA)GTACCTGTAGGGAGAGCAGGGGAGAGAGAAC NGG 10 8 17 8 24 0 100 5 10