Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606182049_egl3 GGGCAGTTTTACCCAGAGCCCTTGACTCTAGG(+AAAAAA)GATCCCTTAGTATTGAGAGACAGGGTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240606182047_3cud TTCCACACAGAATTATCAGAGATAGGCTATCG(+AAAAAA)GATCCTCTTAACTCTGTCTGCTGTTGGAGCT NGG 10 8 17 8 24 0 100 5 10
    240606182046_o147 TAGAAAGCTCCAACAGCAGACAGAGTTAAGAG(+AAAAAA)GATCCGATAGCCTATCTCTGATAATTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240606182044_vko1 TACAGAGACCTGGGTGACCTTGACCTCATAGG(+AAAAAA)GATCCACCTGGTTTTGTTTTTTGTTTTTTGT NGG 10 8 17 8 24 0 100 5 10
    240606182042_392r AACCCACAAAAAACAAAAAACAAAACCAGGTG(+AAAAAA)GATCCCTATGAGGTCAAGGTCACCCAGGTCT NGG 10 8 17 8 24 0 100 5 10
    240606182040_sjps AGGGTGTCCCCTCTGAAGAATGCCTGGGTGGG(+AAAAAA)GATCCTCAGGGACAACTCCTGCAGGGGTGCA NGG 10 8 17 8 24 0 100 5 10
    240606182039_szse CTGCCTGCACCCCTGCAGGAGTTGTCCCTGAG(+AAAAAA)GATCCCCACCCAGGCATTCTTCAGAGGGGAC NGG 10 8 17 8 24 0 100 5 10
    240606182037_t36k CCCAAATTCTTCCTTCCATGTAGCTTCACCTG(+AAAAAA)GATCCCTTGTGTATTTGAGCTTCAGTTACAG NGG 10 8 17 8 24 0 100 5 10
    240606182035_kfm5 AATCTCTGTAACTGAAGCTCAAATACACAAGG(+AAAAAA)GATCCAGGTGAAGCTACATGGAAGGAAGAAT NGG 10 8 17 8 24 0 100 5 10
    240606182034_313q AGCCTAACATTAATTAAAACTTTAAGAAAATG(+AAAAAA)GTACCTAAGGGCCAAATGAAGTTAGAGTTGA NGG 10 8 17 8 24 0 100 5 10