Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606182153_7xdd GGGCACAGCCATTTTTGAATTGGACACTAGTG(+AAAAAA)GATCCTTTGAGTACGGTTCGTTTTGACACAC NGG 10 8 17 8 24 0 100 5 10
    240606182152_a31s TGGATGCTCTCTCCTTAACTGTGGACTTGCAG(+AAAAAA)GATCCACATATAGACACTTCAAAGACTTTCC NGG 10 8 17 8 24 0 100 5 10
    240606182151_rb6y CACAGGGAAAGTCTTTGAAGTGTCTATATGTG(+AAAAAA)GATCCTGCAAGTCCACAGTTAAGGAGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606182150_js3y CACAGGGAAAGTCTTTGAAGTGTCTATATGTG(+AAAAAA)GATCCTGCAAGTCCACAGTTAAGGAGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606182148_wnf0 TGGATGCTCTCTCCTTAACTGTGGACTTGCAG(+AAAAAA)GATCCACATATAGACACTTCAAAGACTTTCC NGG 10 8 17 8 24 0 100 5 10
    240606182147_4vrt CACTTAGGTCAGTGTATCAGGCTTGGTGGCAG(+AAAAAA)GTACCTGTTCATGCTGAGGCATCGTGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606182146_duoo GTGAGGCTGGCACGATGCCTCAGCATGAACAG(+AAAAAA)GTACCTGCCACCAAGCCTGATACACTGACCT NGG 10 8 17 8 24 0 100 5 10
    240606182144_g6yu ATGGCCTTGACTCCTTTGTGGCTGAGCTGTTG(+AAAAAA)GTACCAGTGCAGTTCACGGGTGTTAGGACTG NGG 10 8 17 8 24 0 100 5 10
    240606182143_bzjq TCCTCCAGTCCTAACACCCGTGAACTGCACTG(+AAAAAA)GTACCAACAGCTCAGCCACAAAGGAGTCAAG NGG 10 8 17 8 24 0 100 5 10
    240606182142_46rg TCAAGGAAGTAGATAATCAACAGTGGGAACTG(+AAAAAA)GTACCCTCTGTCCCCAGGCCTGATTTAGAAG NGG 10 8 17 8 24 0 100 5 10