Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606182256_tynd TAGCTAAATTGACTGTCCAGGAAAGCCCTCAG(+AAAAAA)GATCCCACTGCATACACGTCTCAGCACAAGA NGG 10 8 17 8 24 0 100 5 10
    240606182255_3d0w ACATGATTACATATATTTGGCAAGTGCACTTG(+AAAAAA)GATCCTCCTTACCTGACTGATGAACAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606182254_97e2 TTATTTTTCTTGTTCATCAGTCAGGTAAGGAG(+AAAAAA)GATCCAAGTGCACTTGCCAAATATATGTAAT NGG 10 8 17 8 24 0 100 5 10
    240606182252_po12 TCAGCTGGTATAGAATATGCCTCAAACGCATG(+AAAAAA)GATCCCAGGTTTGATCTCAAATGCAGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240606182250_c69d GTGCCACACTCTGCATTTGAGATCAAACCTGG(+AAAAAA)GATCCATGCGTTTGAGGCATATTCTATACCA NGG 10 8 17 8 24 0 100 5 10
    240606182248_3m36 GACCATTGTTACTGGCTGAATTAATCTCCAGG(+AAAAAA)GTACCTGGAATATTTGAACTGGGGCATGACC NGG 10 8 17 8 24 0 100 5 10
    240606182246_mf55 TCCCAGGTCATGCCCCAGTTCAAATATTCCAG(+AAAAAA)GTACCCTGGAGATTAATTCAGCCAGTAACAA NGG 10 8 17 8 24 0 100 5 10
    240606182245_dmow ATGGTGCCCATCAATGGCGTTCCTGCATGGTG(+AAAAAA)GTACCAGTCTCAGGCTTTGGGTGCAAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240606182243_xcho TCTCTGTCCTTTGCACCCAAAGCCTGAGACTG(+AAAAAA)GTACCACCATGCAGGAACGCCATTGATGGGC NGG 10 8 17 8 24 0 100 5 10
    240606182242_g4b5 ATGCACTAACTTTCAAGTCAGTGTGCTGATTG(+AAAAAA)GTACCTAAAAAGCTCACCACATTTGCAGAAG NGG 10 8 17 8 24 0 100 5 10