Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606182357_76gz CTTCCCCAGCTCCTCCCCTCGGATGACTCTTG(+AAAAAA)GTACCCAGGGAAGGTGCTGGGGGCTGGAGCT NGG 10 8 17 8 24 0 100 5 10
    240606182354_wha5 CCCATTCGAGCAAATGGCCTGATGGTCCCCAG(+AAAAAA)GATCCTTGGTGGAAGTAGGCAGTGTGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240606182352_5rmj TGACTTCCTGCACACTGCCTACTTCCACCAAG(+AAAAAA)GATCCTGGGGACCATCAGGCCATTTGCTCGA NGG 10 8 17 8 24 0 100 5 10
    240606182350_kz6f TCTGGTGGGGGGCTATGCTGGAGATGAAAGGG(+AAAAAA)GATCCCAGCATGAGCCTGGAGGTCCCTGGCA NGG 10 8 17 8 24 0 100 5 10
    240606182348_isc4 TCCACTGCCAGGGACCTCCAGGCTCATGCTGG(+AAAAAA)GATCCCCTTTCATCTCCAGCATAGCCCCCCA NGG 10 8 17 8 24 0 100 5 10
    240606182347_l1wb ACGCTGGTGGAATCCTACGTATTCAAAATTAG(+AAAAAA)GATCCTTGTGTAGCACTGAACCTGGGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606182346_s2hf AAATCTCTTTCCCAGGTTCAGTGCTACACAAG(+AAAAAA)GATCCTAATTTTGAATACGTAGGATTCCACC NGG 10 8 17 8 24 0 100 5 10
    240606182344_3wvb CTGTTTCACAAGCATCTGCTCTTGGCCTTTCG(+AAAAAA)GTACCACCTCCTGATTCTCAACCACCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606182343_zow8 CCAGACCCCAGGTGGTTGAGAATCAGGAGGTG(+AAAAAA)GTACCGAAAGGCCAAGAGCAGATGCTTGTGA NGG 10 8 17 8 24 0 100 5 10
    240606182341_0pv0 AAATAATTATGGCCACAAAATTATATGTGATG(+AAAAAA)GATCCACTGCTTTTTTATATTCTTTTCAATA NGG 10 8 17 8 24 0 100 5 10