Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606182458_wce1 GTTATGTGATAATTTTAATCTTGGAATGAATG(+AAAAAA)GATCCAATATGTAGATTGTTCATTTTATAGT NGG 10 8 17 8 24 0 100 5 10
    240606182456_liii ACTTGCATGAGTTGATTCTCTCTTACCGCGTG(+AAAAAA)GATCCCTGGGATTGAATTCAGGCCACCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606182454_g4vh CACGAGCCTGGTGGCCTGAATTCAATCCCAGG(+AAAAAA)GATCCACGCGGTAAGAGAGAATCAACTCATG NGG 10 8 17 8 24 0 100 5 10
    240606182452_ouzx ACCTCCAAGAAGCAGGCTTGATGGTCAGAAAG(+AAAAAA)GATCCTTGAGGCCAAGGAAGGATCTTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240606182451_6wws GACCCCAACAAAGATCCTTCCTTGGCCTCAAG(+AAAAAA)GATCCTTTCTGACCATCAAGCCTGCTTCTTG NGG 10 8 17 8 24 0 100 5 10
    240606182449_ks5a AGGGGGGCATGCAATATGGGTGAGGGAGGGCG(+AAAAAA)GTACCCTTCTCATCCCAGACCTGAGCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240606182447_mvta GGGAGGGGGGGCTCAGGTCTGGGATGAGAAGG(+AAAAAA)GTACCGCCCTCCCTCACCCATATTGCATGCC NGG 10 8 17 8 24 0 100 5 10
    240606182445_yn5g AGGAGAGAAGATGGACACAAGGGCAGATGAGG(+AAAAAA)GATCCAGACAAGAAGATAGAGTTCCTGCTAT NGG 10 8 17 8 24 0 100 5 10
    240606182444_hs28 GAACAATAGCAGGAACTCTATCTTCTTGTCTG(+AAAAAA)GATCCCTCATCTGCCCTTGTGTCCATCTTCT NGG 10 8 17 8 24 0 100 5 10
    240606182443_2oq8 GCAGGACAATGCACCTTGTCCGGGGCTCATAG(+AAAAAA)GATCCAGGAGTGCCCCGAGTTGACAACGAAG NGG 10 8 17 8 24 0 100 5 10