Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606182530_tmlo GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+AAAAAA)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606182529_jywk CTTTAAATGATGTGGAAATGGACAGACTCTTG(+AAAAAA)GATCCCATGGCTTAATTTTGAACAGGGGAAC NGG 10 8 17 8 24 0 100 5 10
    240606182527_y0tn GCCTAGTTCCCCTGTTCAAAATTAAGCCATGG(+AAAAAA)GATCCAAGAGTCTGTCCATTTCCACATCATT NGG 10 8 17 8 24 0 100 5 10
    240606182525_51s3 GAGCCCATCCCTGGCACTGCCTGGGGGGCTAG(+AAAAAA)GATCCAGAGGGTGGATAGCCCAGAGGCCTAG NGG 10 8 17 8 24 0 100 5 10
    240606182523_3lvp CTATCCTAGGCCTCTGGGCTATCCACCCTCTG(+AAAAAA)GATCCTAGCCCCCCAGGCAGTGCCAGGGATG NGG 10 8 17 8 24 0 100 5 10
    240606182521_f3gt TTCTTAACACTGACCATATTCGGGGGTGGGGG(+AAAAAA)GATCCAAAAATTACAAACCATTTACTAGTTG NGG 10 8 17 8 24 0 100 5 10
    240606182519_owyw ATTTTCAACTAGTAAATGGTTTGTAATTTTTG(+AAAAAA)GATCCCCCCACCCCCGAATATGGTCAGTGTT NGG 10 8 17 8 24 0 100 5 10
    240606182517_d9ja TTGTTGTTTTTTTTTTTTCTTACTAAAAGTGG(+AAAAAA)GATCCTTTTAGGCAGGATTTTATTCTGTAGC NGG 10 8 17 8 24 0 100 5 10
    240606182516_0grk CTTTGGCTACAGAATAAAATCCTGCCTAAAAG(+AAAAAA)GATCCCACTTTTAGTAAGAAAAAAAAAAAAC NGG 10 8 17 8 24 0 100 5 10
    240606182515_grsm TAACCTCAGCCCTGAGTTCAGGGAGACAGGTG(+AAAAAA)GATCCCATGGACTCACTGGCTGGATAAGCTA NGG 10 8 17 8 24 0 100 5 10