Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606182226_eciv GTGAAAAAATGTGGGAGTAGAAAGAAAAATGG(+AAAAAA)GATCCATGTGCTTCCAATTTAAAATTTATGG NGG 10 8 17 8 24 0 100 5 10
    240606182224_gmwr GGCGGGAAACATGGAGGCCCGGCGGTAAGGGG(+AAAAAA)GATCCCGGGCGGGCCAATCTACAAATACGCG NGG 10 8 17 8 24 0 100 5 10
    240606182220_8695 GGGGACGCGTATTTGTAGATTGGCCCGCCCGG(+AAAAAA)GATCCCCCTTACCGCCGGGCCTCCATGTTTC NGG 10 8 17 8 24 0 100 5 10
    240606182219_o289 CCAACAGGGAAAAGTCACCTGACTCCATTTAG(+AAAAAA)GTACCCCAAAATTTCCCAACCATGTTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240606182217_9nw9 GGTGTATAGAAACATGGTTGGGAAATTTTGGG(+AAAAAA)GTACCTAAATGGAGTCAGGTGACTTTTCCCT NGG 10 8 17 8 24 0 100 5 10
    240606182215_cc0z GTGGCATGCTTAGGGAAACTCTGGAAAGCTCG(+AAAAAA)GTACCATGCAAAGTGCCTGGCCCAGAACTAA NGG 10 8 17 8 24 0 100 5 10
    240606182214_ajxo GAAACTTAGTTCTGGGCCAGGCACTTTGCATG(+AAAAAA)GTACCGAGCTTTCCAGAGTTTCCCTAAGCAT NGG 10 8 17 8 24 0 100 5 10
    240606182213_j96o CCGGGATATACTCTCATTCTCTCTTCTTCAAG(+AAAAAA)GTACCTGCATGTGTGTGTGTGTGTGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606182211_bjqi ACACACACACACACACACACACACACATGCAG(+AAAAAA)GTACCTTGAAGAAGAGAGAATGAGAGTATAT NGG 10 8 17 8 24 0 100 5 10
    240606182209_e5fd AGCACTGAGGTTGTGCTTCATTGTTGAATTTG(+AAAAAA)GTACCACATGTGAAGAAATGATCAAATCAAA NGG 10 8 17 8 24 0 100 5 10