Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606182103_o532 TTTCTGTTCTCTCTCCCCTGCTCTCCCTACAG(+AAAAAA)GTACCTACACACACACACACACACACATCCC NGG 10 8 17 8 24 0 100 5 10
    240606182102_0l9f GGCACAGGCTAACAGAATGGATACTTAAATAG(+AAAAAA)GATCCATCATTTTGTTGCATACAAGAAACAC NGG 10 8 17 8 24 0 100 5 10
    240606182101_hkls GAAGTGTGTTTCTTGTATGCAACAAAATGATG(+AAAAAA)GATCCTATTTAAGTATCCATTCTGTTAGCCT NGG 10 8 17 8 24 0 100 5 10
    240606182059_pts0 AGAAGATTATACCGTCTCCTCAGCACTTTGTG(+AAAAAA)GATCCTTCTCCAAAATTGACCATATACTCAA NGG 10 8 17 8 24 0 100 5 10
    240606182058_rq99 TATGATTGAGTATATGGTCAATTTTGGAGAAG(+AAAAAA)GATCCACAAAGTGCTGAGGAGACGGTATAAT NGG 10 8 17 8 24 0 100 5 10
    240606182056_8kf2 GGGTCTGTCCCTGAATCTGTTGCCTGCCTGTG(+AAAAAA)GATCCCATTCCCTTAACTTGTCTGGTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606182055_pwyy TCCCACTGAGACCAGACAAGTTAAGGGAATGG(+AAAAAA)GATCCACAGGCAGGCAACAGATTCAGGGACA NGG 10 8 17 8 24 0 100 5 10
    240606182053_q8t3 AAGCCAACTAGAAAACAACTGGCGTTTATCAG(+AAAAAA)GTACCTTAGATTGGTCACTTATACAGCTAAT NGG 10 8 17 8 24 0 100 5 10
    240606182052_j2q4 ATAAAATTAGCTGTATAAGTGACCAATCTAAG(+AAAAAA)GTACCTGATAAACGCCAGTTGTTTTCTAGTT NGG 10 8 17 8 24 0 100 5 10
    240606182050_q1xv ACACAGAGCAACCCTGTCTCTCAATACTAAGG(+AAAAAA)GATCCCTAGAGTCAAGGGCTCTGGGTAAAAC NGG 10 8 17 8 24 0 100 5 10