Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606181220_154t TCACTGTGTAGCCCTGGCCTCAAACTCACAGG(+AAAAAA)GATCCACCTGCCTCTGCCTCCTGAGAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240606181218_llnx TGTTAGCATCGGAAATTGAGGCAAAACCATTG(+AAAAAA)GATCCAAGAAGCCAGGCCTGTTTCTTTAACA NGG 10 8 17 8 24 0 100 5 10
    240606181216_nomf ATAGTTGTTAAAGAAACAGGCCTGGCTTCTTG(+AAAAAA)GATCCAATGGTTTTGCCTCAATTTCCGATGC NGG 10 8 17 8 24 0 100 5 10
    240606181215_s6hy TAGGAAGATATTACCAACCCTGCTCAAACAGG(+AAAAAA)GTACCAAAGGAACGGTTAAGGTCCCCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240606181213_u7fn GGTTAAGAGAGGGGACCTTAACCGTTCCTTTG(+AAAAAA)GTACCCTGTTTGAGCAGGGTTGGTAATATCT NGG 10 8 17 8 24 0 100 5 10
    240606181212_qwt1 TGTTCCAAGACACACCCCTTCTAGAGATGCTG(+AAAAAA)GATCCAACCCAAACCTGCCCACTCCGGAACC NGG 10 8 17 8 24 0 100 5 10
    240606181210_6iyb TGGTGGGTTCCGGAGTGGGCAGGTTTGGGTTG(+AAAAAA)GATCCAGCATCTCTAGAAGGGGTGTGTCTTG NGG 10 8 17 8 24 0 100 5 10
    240606181208_c00k TATTGGATGGGCACTGGGGGCCAGGGGCCTTG(+AAAAAA)GATCCCAAATAAAAGAGAAGTTCTTCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240606181206_wtnn GTGGCCCACAGAAGAACTTCTCTTTTATTTGG(+AAAAAA)GATCCAAGGCCCCTGGCCCCCAGTGCCCATC NGG 10 8 17 8 24 0 100 5 10
    240606181204_q934 TATTGGATGGGCACTGGGGGCCAGGGGCCTTG(+AAAAAA)GATCCCAAATAAAAGAGAAGTTCTTCTGTGG NGG 10 8 17 8 24 0 100 5 10