Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606181114_ntwi ATTTGGCTTTAAAAACTACTCGGCCCAAGAGG(+AAAAAA)GATCCAGGGAGTAGCTCCCACTGTTGGCAGT NGG 10 8 17 8 24 0 100 5 10
    240606181113_ok1w TGGTGGAACCTGTACACTGAAGAGTTAGTGAG(+AAAAAA)GTACCCCAAATTACAGCAGTACCCCCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240606181111_y9bb CTCCAGAGAAGGGGGTACTGCTGTAATTTGGG(+AAAAAA)GTACCTCACTAACTCTTCAGTGTACAGGTTC NGG 10 8 17 8 24 0 100 5 10
    240606181109_hmrr CAATATCACGACGTGGAAATAGGAAGTTTGAG(+AAAAAA)GATCCCCAGAGCAGCCAGCCTTCACGGGTTC NGG 10 8 17 8 24 0 100 5 10
    240606181107_7tkk CCCAGGAACCCGTGAAGGCTGGCTGCTCTGGG(+AAAAAA)GATCCTCAAACTTCCTATTTCCACGTCGTGA NGG 10 8 17 8 24 0 100 5 10
    240606181105_s6n9 AGGCTACGTGCAGTATCTGCAATTCACGGATG(+AAAAAA)GATCCATCACTTTGGGTCTGAGGTACGGCTA NGG 10 8 17 8 24 0 100 5 10
    240606181103_3qry ATGTTTAGCCGTACCTCAGACCCAAAGTGATG(+AAAAAA)GATCCATCCGTGAATTGCAGATACTGCACGT NGG 10 8 17 8 24 0 100 5 10
    240606181102_daqg GTACAATGGTGGCTTTAGCAACCACAAAGCTG(+AAAAAA)GATCCAGAAGTACTCATTAGTGTTGTATTTG NGG 10 8 17 8 24 0 100 5 10
    240606181101_oeee AGATGCAAATACAACACTAATGAGTACTTCTG(+AAAAAA)GATCCAGCTTTGTGGTTGCTAAAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606181100_hc0n GAAGGAAGAGGATACGGATCACAAGGTCTCTG(+AAAAAA)GATCCATGGATTTCTGACTGCCTTGTCTAAA NGG 10 8 17 8 24 0 100 5 10