Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606181147_kf4d TTTTAAATTACTTTTAAAAAATATTAAGATGG(+AAAAAA)GATCCTAGCATTTTGTTTATAGAGTACAAAG NGG 10 8 17 8 24 0 100 5 10
    240606181146_j2d5 TGTAAGATATGGGGACACAATACATTTCACTG(+AAAAAA)GTACCATGAGGAAGGGTGTTAACACTAAATT NGG 10 8 17 8 24 0 100 5 10
    240606181144_6ehu ACTCCAATTTAGTGTTAACACCCTTCCTCATG(+AAAAAA)GTACCAGTGAAATGTATTGTGTCCCCATATC NGG 10 8 17 8 24 0 100 5 10
    240606181142_l38w CTCACCCTGAGAAAGGTTTGGTGCAACCCTGG(+AAAAAA)GATCCCTAACACCAAGCACTACTGCCTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606181140_u8hu TTAGCCAGCAGGCAGTAGTGCTTGGTGTTAGG(+AAAAAA)GATCCCAGGGTTGCACCAAACCTTTCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240606181139_e4xm CTCTAGAGAACCCTGACTAATAACAGAAGTTG(+AAAAAA)GTACCAGGAGTGGTTCTAGAGTAACAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240606181137_t0ds CTTGTACTTCTGTTACTCTAGAACCACTCCTG(+AAAAAA)GTACCAACTTCTGTTATTAGTCAGGGTTCTC NGG 10 8 17 8 24 0 100 5 10
    240606181136_m49h TCCTCAGAAAATTGGACATAGTACTACCAGAG(+AAAAAA)GATCCCGCAATGTCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240606181134_o15g TTCTGGATATATGCCCAGGAGAGACATTGCGG(+AAAAAA)GATCCTCTGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606181132_xjyn GGATGGACCATCTAGAGACTGCCATATCTGGG(+AAAAAA)GATCCATCCCATAATCAGCCTCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10