Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606181203_680d GTGGCCCACAGAAGAACTTCTCTTTTATTTGG(+AAAAAA)GATCCAAGGCCCCTGGCCCCCAGTGCCCATC NGG 10 8 17 8 24 0 100 5 10
    240606181201_dnzs ATTGCGGACCATGGATATATTTTATTAACTGG(+AAAAAA)GTACCCAAAACACATTTCCCATATTTGGATG NGG 10 8 17 8 24 0 100 5 10
    240606181159_r8b3 TTCACCATCCAAATATGGGAAATGTGTTTTGG(+AAAAAA)GTACCCAGTTAATAAAATATATCCATGGTCC NGG 10 8 17 8 24 0 100 5 10
    240606181158_7t0b TGGTAGTGTTCAGCGTTGCCGTCAAGTAGAGG(+AAAAAA)GTACCTCAAAATATATGGTCCTTGTCCCTCG NGG 10 8 17 8 24 0 100 5 10
    240606181157_6epe GAGAACGAGGGACAAGGACCATATATTTTGAG(+AAAAAA)GTACCCTCTACTTGACGGCAACGCTGAACAC NGG 10 8 17 8 24 0 100 5 10
    240606181156_vobx TGGGAAAGACTCCTCTGAGAAAATTTAAGATG(+AAAAAA)GATCCCAACTCTGTGAGTGTGTCTGTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240606181154_2wz5 GAATACCTGCACAGACACACTCACAGAGTTGG(+AAAAAA)GATCCATCTTAAATTTTCTCAGAGGAGTCTT NGG 10 8 17 8 24 0 100 5 10
    240606181153_k31t CTCTAGTGGAGGCTTGGTATTAGAAAAGTCTG(+AAAAAA)GTACCCCTTAGGCACCTTTCTTGGCATATGA NGG 10 8 17 8 24 0 100 5 10
    240606181150_toi6 TAACCTCATATGCCAAGAAAGGTGCCTAAGGG(+AAAAAA)GTACCAGACTTTTCTAATACCAAGCCTCCAC NGG 10 8 17 8 24 0 100 5 10
    240606181148_aspq GAGCCCTTTGTACTCTATAAACAAAATGCTAG(+AAAAAA)GATCCCATCTTAATATTTTTTAAAAGTAATT NGG 10 8 17 8 24 0 100 5 10