Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606181058_znqu ACAGCTTTAGACAAGGCAGTCAGAAATCCATG(+AAAAAA)GATCCAGAGACCTTGTGATCCGTATCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240606181056_4kon AAAAATCAAAGCACTCATTCACTTGGGGGGAG(+AAAAAA)GATCCTACGCAGGGGGGACTTGGTGCGTATA NGG 10 8 17 8 24 0 100 5 10
    240606181055_5fbc CTTAATATACGCACCAAGTCCCCCCTGCGTAG(+AAAAAA)GATCCTCCCCCCAAGTGAATGAGTGCTTTGA NGG 10 8 17 8 24 0 100 5 10
    240606181053_owgu GAGAAGGCCCTGTCTCCTCCTCAAGCCAAAGG(+AAAAAA)GATCCCAGCAGCAGCATCGTGGTGGCATCTT NGG 10 8 17 8 24 0 100 5 10
    240606181052_l0xm CTCCAAAGATGCCACCACGATGCTGCTGCTGG(+AAAAAA)GATCCCTTTGGCTTGAGGAGGAGACAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240606181050_oydb ACACACACACACACACACACTCTTAGCAGAAG(+AAAAAA)GTACCTCATAGTCTTTCCTAGTCGTCTCATA NGG 10 8 17 8 24 0 100 5 10
    240606181049_ssoq AAATGTATGAGACGACTAGGAAAGACTATGAG(+AAAAAA)GTACCTTCTGCTAAGAGTGTGTGTGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606181047_2ph0 GACTACTTTCTGATGGCCCCTGTTCTTGCGGG(+AAAAAA)GATCCTGACAGTAAGACACGGTTGGGAACTA NGG 10 8 17 8 24 0 100 5 10
    240606181046_7e31 CCCAGTAGTTCCCAACCGTGTCTTACTGTCAG(+AAAAAA)GATCCCCGCAAGAACAGGGGCCATCAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606181045_m46c TGTGGATTTGTTATATTTTAATATGTGAAATG(+AAAAAA)GATCCAGTCATTGGAACAGATGTGTGAAGCT NGG 10 8 17 8 24 0 100 5 10