Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606173828_qk8x GAAGCTACCTTGTGGATGCTAGGAATCAAACG(+AAAAAA)GATCCTCTGCACAAGAGGCCAGTGCTCTTAG NGG 10 8 17 8 24 0 100 5 10
    240606173826_txln TGTCCACCCACTTTCATGACAACCCAATTTGG(+AAAAAA)GATCCCCATGGCAAGGGTGGGGTAGTACAGA NGG 10 8 17 8 24 0 100 5 10
    240606173824_ednq TTATTTCTGTACTACCCCACCCTTGCCATGGG(+AAAAAA)GATCCCAAATTGGGTTGTCATGAAAGTGGGT NGG 10 8 17 8 24 0 100 5 10
    240606173822_lddn GACTAACACCTTCTATAACCCCAGCCCCTAGG(+AAAAAA)GATCCGACAACCTCTAGCTTCCGCGGGCACC NGG 10 8 17 8 24 0 100 5 10
    240606173820_rgex GTTCAGGTGCCCGCGGAAGCTAGAGGTTGTCG(+AAAAAA)GATCCCTAGGGGCTGGGGTTATAGAAGGTGT NGG 10 8 17 8 24 0 100 5 10
    240606173819_5xvq GGTACTGCTCAGGCTAATAAGAAGTGAAGTCG(+AAAAAA)GATCCAGAGCCTGATTCCCTTGCAAGCCAGT NGG 10 8 17 8 24 0 100 5 10
    240606173818_foc8 TGCGCACTGGCTTGCAAGGGAATCAGGCTCTG(+AAAAAA)GATCCGACTTCACTTCTTATTAGCCTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606173817_zie3 AAGCCCATTCCACAGAAGTGAGTTCACATTTG(+AAAAAA)GTACCGTGAGCCTAGTCAAAAGCTTGCAGCT NGG 10 8 17 8 24 0 100 5 10
    240606173815_ie5q TCCCTAGCTGCAAGCTTTTGACTAGGCTCACG(+AAAAAA)GTACCAAATGTGAACTCACTTCTGTGGAATG NGG 10 8 17 8 24 0 100 5 10
    240606173814_y8gw AGCATCTTCACTCTTCTGGCCTACCAAGCTTG(+AAAAAA)GTACCTATTTCTCATCATAACTTTGGAATGT NGG 10 8 17 8 24 0 100 5 10