Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606173727_d18b ATCACTAATTGTAGAAAATATAGATATTCAAG(+AAAAAA)GATCCACTGATCACCTCAATACATGGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606173725_9l1v TAGCTGGACTCCATAACTCTGACCACTGGGAG(+AAAAAA)GATCCTTAGCAGCCCCTGCTTGCCGGGTGAC NGG 10 8 17 8 24 0 100 5 10
    240606173724_fq0p GTGCTGTCACCCGGCAAGCAGGGGCTGCTAAG(+AAAAAA)GATCCTCCCAGTGGTCAGAGTTATGGAGTCC NGG 10 8 17 8 24 0 100 5 10
    240606173723_f7a2 AATCTAGGACCGGATGAGATGGACGGTGTTTG(+AAAAAA)GATCCTCTTGCTTTTACTCTCACTCTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240606173722_ik49 CTCAATTTTAAGAGTGAGAGTAAAAGCAAGAG(+AAAAAA)GATCCAAACACCGTCCATCTCATCCGGTCCT NGG 10 8 17 8 24 0 100 5 10
    240606173721_z2j9 CCCCGTGACTGTGTGGAGCAAAGCCGGGGCAG(+AAAAAA)GTACCCGAGCTTTGCGATCCCAACAGGAGCG NGG 10 8 17 8 24 0 100 5 10
    240606173719_k2wl CAGCTCGCTCCTGTTGGGATCGCAAAGCTCGG(+AAAAAA)GTACCTGCCCCGGCTTTGCTCCACACAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606173717_0ixx AGACAGCTGTTCCCTATCTGCAGGTGGCCTAG(+AAAAAA)GTACCACTGTGAAGACAGATGCCTGTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606173716_vfot TTCTTAGGAAACAGGCATCTGTCTTCACAGTG(+AAAAAA)GTACCTAGGCCACCTGCAGATAGGGAACAGC NGG 10 8 17 8 24 0 100 5 10
    240606173714_tvvt TCTTGGGTTGGGGAGATGAGACCCGAGTTTAG(+AAAAAA)GTACCACCCAAACATACAGAACAAAGGAAGG NGG 10 8 17 8 24 0 100 5 10