Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606173640_3cui CAAAGGGCAGGAGGCTCTGTACCTAGAAGCAG(+AAAAAA)GTACCATTCCACAGGTGGCATGAGGCTTCAG NGG 10 8 17 8 24 0 100 5 10
    240606173638_fz36 CTACAGCCAGAGCCAGTGGGGTCACGACCACG(+AAAAAA)GATCCTGTGCAGAAGAGGTGGGGATCATTCG NGG 10 8 17 8 24 0 100 5 10
    240606173637_lid4 GTCCACGAATGATCCCCACCTCTTCTGCACAG(+AAAAAA)GATCCGTGGTCGTGACCCCACTGGCTCTGGC NGG 10 8 17 8 24 0 100 5 10
    240606173635_yocf AAGGTCAGGCCAGGCTACAGCAGGGCATCCTG(+AAAAAA)GTACCCAATCAGTGTGGCCGCCTTCCTGGCC NGG 10 8 17 8 24 0 100 5 10
    240606173633_vdmd TGAAGGGCCAGGAAGGCGGCCACACTGATTGG(+AAAAAA)GTACCAGGATGCCCTGCTGTAGCCTGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240606173632_0rhe CCCCACAGAGAACATGAAGTCACAGTGGAGTG(+AAAAAA)GATCCCCTGTTTCTTAGGTATTTGATCAGAG NGG 10 8 17 8 24 0 100 5 10
    240606173630_afvy GAACTCTCTGATCAAATACCTAAGAAACAGGG(+AAAAAA)GATCCACTCCACTGTGACTTCATGTTCTCTG NGG 10 8 17 8 24 0 100 5 10
    240606173629_zfbe GAAACTGAGGGAATTTGTGGCCTTAAAGCAGG(+AAAAAA)GATCCCAGAGTAAAGCTCTCAGGGTTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606173627_79ly AAGGACCCAGAACCCTGAGAGCTTTACTCTGG(+AAAAAA)GATCCCTGCTTTAAGGCCACAAATTCCCTCA NGG 10 8 17 8 24 0 100 5 10
    240606173626_mmpm GAGGCTGGCGGAGGCTGGTGTCTCATCATCGG(+AAAAAA)GATCCCCTCCCCAGGACCAGGAGGGCTGCAG NGG 10 8 17 8 24 0 100 5 10