Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606173740_5lj8 CCATGACCCTGGTGCCTAACCATAATGTCCAG(+AAAAAA)GATCCAGAAAGAACACTTGGTTGTATAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606173739_eh66 GCTTAAGATTTTGCTACAGAGACAAAAAGGTG(+AAAAAA)GATCCTTTCCCTGCCCAGCTACTCTGTCCAC NGG 10 8 17 8 24 0 100 5 10
    240606173738_kj0n CCCTGGTGGACAGAGTAGCTGGGCAGGGAAAG(+AAAAAA)GATCCACCTTTTTGTCTCTGTAGCAAAATCT NGG 10 8 17 8 24 0 100 5 10
    240606173737_u802 AGTGGTTAAGAGCACTGACTGTTCTTAGAGAG(+AAAAAA)GATCCAGGTTTGATTCCCAACACCCACATGG NGG 10 8 17 8 24 0 100 5 10
    240606173735_d9sd GCTTTCCATGTGGGTGTTGGGAATCAAACCTG(+AAAAAA)GATCCTCTCTAAGAACAGTCAGTGCTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240606173734_azec ATTCTGCCATGTGGGTGCTTACCTGTGCTGAG(+AAAAAA)GTACCAAGCACCTGTCTAGTTACATCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606173733_hv15 TGCCCAGGAAGATGTAACTAGACAGGTGCTTG(+AAAAAA)GTACCTCAGCACAGGTAAGCACCCACATGGC NGG 10 8 17 8 24 0 100 5 10
    240606173731_wttz GGTGGAGACTAATATAGCTGTCTCATTTAGAG(+AAAAAA)GTACCCATTCAGTCTCTATAGCCCTTTGACC NGG 10 8 17 8 24 0 100 5 10
    240606173730_cdqh CAACTGGTCAAAGGGCTATAGAGACTGAATGG(+AAAAAA)GTACCTCTAAATGAGACAGCTATATTAGTCT NGG 10 8 17 8 24 0 100 5 10
    240606173728_3h5q GTCCACTTCTCCATGTATTGAGGTGATCAGTG(+AAAAAA)GATCCTTGAATATCTATATTTTCTACAATTA NGG 10 8 17 8 24 0 100 5 10