Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606173813_rvyr ACCCTACATTCCAAAGTTATGATGAGAAATAG(+AAAAAA)GTACCAAGCTTGGTAGGCCAGAAGAGTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606173811_0ckc ACATGCCTTTGGGTGTCTGTTTGCCAGGCTGG(+AAAAAA)GATCCCGGGCTCCTGAGTGAGCAGTAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606173808_lxjp ACAGTCCAGCTACTGCTCACTCAGGAGCCCGG(+AAAAAA)GATCCCAGCCTGGCAAACAGACACCCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606173807_mwkg AAGATAACCAAGGCTTCCACTGGCAGGGTTTG(+AAAAAA)GATCCCAGGCAGGCAAGCAGGCAGGCTGGTG NGG 10 8 17 8 24 0 100 5 10
    240606173804_qipy CCATCCACCAGCCTGCCTGCTTGCCTGCCTGG(+AAAAAA)GATCCAAACCCTGCCAGTGGAAGCCTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240606173802_el3c CTGCCTCAGCCTGTGCTCCAGGTTGGTTCCAG(+AAAAAA)GTACCCTAGCGGCCTTGGCAGGTTTTGCAGC NGG 10 8 17 8 24 0 100 5 10
    240606173801_2rig GATGTGCTGCAAAACCTGCCAAGGCCGCTAGG(+AAAAAA)GTACCTGGAACCAACCTGGAGCACAGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606173759_yz7v TAATGAGCTTCCTTTTCTCCAATGTGTATGAG(+AAAAAA)GTACCTGTATGATGTGCTCTGATCACACCCA NGG 10 8 17 8 24 0 100 5 10
    240606173758_rwok AAGGGTGGGTGTGATCAGAGCACATCATACAG(+AAAAAA)GTACCTCATACACATTGGAGAAAAGGAAGCT NGG 10 8 17 8 24 0 100 5 10
    240606173757_5zfz GTTTCTAACAGAAATTACGATTTGAGGGTCAG(+AAAAAA)GATCCTGGGAGAGACTCTCCAGCTCCAGGAC NGG 10 8 17 8 24 0 100 5 10