Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606173712_btgl TATGGCCTTCCTTTGTTCTGTATGTTTGGGTG(+AAAAAA)GTACCTAAACTCGGGTCTCATCTCCCCAACC NGG 10 8 17 8 24 0 100 5 10
    240606173711_d6sq GGGTATACAATGCCCTCTCCTGTTCTCTGTGG(+AAAAAA)GTACCTGCAGTCATGTGCACATACCTGCACG NGG 10 8 17 8 24 0 100 5 10
    240606173710_enug ACACACGTGCAGGTATGTGCACATGACTGCAG(+AAAAAA)GTACCCACAGAGAACAGGAGAGGGCATTGTA NGG 10 8 17 8 24 0 100 5 10
    240606173708_188k CGTGGTAAGGCGAGCCTTCCTTTGTGGGACAG(+AAAAAA)GATCCTTCACTTCATCTTTTCCAAAAACTGT NGG 10 8 17 8 24 0 100 5 10
    240606173707_prv4 CACACACAGTTTTTGGAAAAGATGAAGTGAAG(+AAAAAA)GATCCTGTCCCACAAAGGAAGGCTCGCCTTA NGG 10 8 17 8 24 0 100 5 10
    240606173705_knbq GAATAGAATACAGGTCCCAAGGCCCACGGTGG(+AAAAAA)GTACCTGAAACTGTGGGCAGCTCCGAGCCCT NGG 10 8 17 8 24 0 100 5 10
    240606173704_tkmb CATATAGGGCTCGGAGCTGCCCACAGTTTCAG(+AAAAAA)GTACCCACCGTGGGCCTTGGGACCTGTATTC NGG 10 8 17 8 24 0 100 5 10
    240606173702_q6t5 GTATCCTGAGCTCAGACTCCGCCCCGTGGAAG(+AAAAAA)GATCCCACGGCACTCTCACTTACATAGGGTG NGG 10 8 17 8 24 0 100 5 10
    240606173700_g7cb TCACTCACCCTATGTAAGTGAGAGTGCCGTGG(+AAAAAA)GATCCTTCCACGGGGCGGAGTCTGAGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606173658_3o5h ACATAGGAGATGAGGCTGGAAGCCATCTTTGG(+AAAAAA)GTACCAGGGTCACTGTACCACCGCTGGCTAC NGG 10 8 17 8 24 0 100 5 10