Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606173534_c8oo GTAGGCCATGGAAGGGGAGAAACCCCTCCCGG(+AAAAAA)GTACCCCCTCTTCTCTGACTGCTGCCCCCTA NGG 10 8 17 8 24 0 100 5 10
    240606173533_8sc0 AGACGCATGCGCACTGGCTTGCAAGGGCTCTG(+AAAAAA)GATCCGACTTCACTTCTTATTAGCCTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606173531_32s6 GGTACTGCTCAGGCTAATAAGAAGTGAAGTCG(+AAAAAA)GATCCAGAGCCCTTGCAAGCCAGTGCGCATG NGG 10 8 17 8 24 0 100 5 10
    240606173529_pzuv GCAGCCCGGCTAGTCCTTGAGATCCCGGCCCG(+AAAAAA)GATCCTGGAGCAGGCGTGCACAGGCCAAAGC NGG 10 8 17 8 24 0 100 5 10
    240606173527_r8gy TGCCAGCTTTGGCCTGTGCACGCCTGCTCCAG(+AAAAAA)GATCCGGGCCGGGATCTCAAGGACTAGCCGG NGG 10 8 17 8 24 0 100 5 10
    240606173526_y0hy AGGATTTGAACTTGGGCCCTCTGTAAGAAGAG(+AAAAAA)GATCCATTCTTAACTCCTGGACCATCTCATT NGG 10 8 17 8 24 0 100 5 10
    240606173525_mv0w AGGCTAATGAGATGGTCCAGGAGTTAAGAATG(+AAAAAA)GATCCTCTTCTTACAGAGGGCCCAAGTTCAA NGG 10 8 17 8 24 0 100 5 10
    240606173523_tq4k ACGAGTGCTGAGGATCTCAGCAGGTGGATGGG(+AAAAAA)GATCCCAGGGATGCAAGAGCTTTCACCCTCT NGG 10 8 17 8 24 0 100 5 10
    240606173520_0tvc GGTGCAGAGGGTGAAAGCTCTTGCATCCCTGG(+AAAAAA)GATCCCCATCCACCTGCTGAGATCCTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606173519_yyrs AAAACAAAAAACCCCAAAACTTCCTACTGCAG(+AAAAAA)GTACCCCTTCATTACAAAAGTATGCAAACCA NGG 10 8 17 8 24 0 100 5 10