Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606173502_14rm GTGAACTCCCGTCTGCAGGAGGATGTATGTGG(+AAAAAA)GTACCTGCTCTGCTGGTATTGTGAAAACAGG NGG 10 8 17 8 24 0 100 5 10
    240606173500_jv49 TTTCTCCATACACACATTTATATTTAGAGAGG(+AAAAAA)GATCCAAACCAGACATGCTGATGTTGGATCT NGG 10 8 17 8 24 0 100 5 10
    240606173459_1nkk ACCTTAGATCCAACATCAGCATGTCTGGTTTG(+AAAAAA)GATCCCTCTCTAAATATAAATGTGTGTATGG NGG 10 8 17 8 24 0 100 5 10
    240606173457_xz9b GTGACATGTACAATATATAGAGGCCCCCACAG(+AAAAAA)GATCCCCTGCTCCTGGACCTGAGCCCAACCA NGG 10 8 17 8 24 0 100 5 10
    240606173455_m3si GCACCTGGTTGGGCTCAGGTCCAGGAGCAGGG(+AAAAAA)GATCCTGTGGGGGCCTCTATATATTGTACAT NGG 10 8 17 8 24 0 100 5 10
    240606173453_li0r TGACCCCAGGAGACCAGAAAGATTGGGCTGGG(+AAAAAA)GATCCAGAACCCACTCCTTAATCCTCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240606173452_24so TGGATCAACTGAGGATTAAGGAGTGGGTTCTG(+AAAAAA)GATCCCCAGCCCAATCTTTCTGGTCTCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606173450_bpi3 GCTTTGTGAAGTTGCTGTTAATAGGCCTTTGG(+AAAAAA)GTACCCTTTGCTTGAAGTTATATCAACTGAG NGG 10 8 17 8 24 0 100 5 10
    240606173448_a5b7 ATCACCTCAGTTGATATAACTTCAAGCAAAGG(+AAAAAA)GTACCCAAAGGCCTATTAACAGCAACTTCAC NGG 10 8 17 8 24 0 100 5 10
    240606173447_vngv CCATATATGTAAGTGCCCCGTGTGTGTGCCTG(+AAAAAA)GTACCGACAGAAGCCAAATGAGTATGTCAGA NGG 10 8 17 8 24 0 100 5 10