Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606173426_n0qa CAAGAGTCCTCAAACTTTGGCAATGACCCCTG(+AAAAAA)GTACCCACTCCTGGTCAAGAGGGCCCCAGTA NGG 10 8 17 8 24 0 100 5 10
    240606173423_byro TGGTCCTTCTCAGGCACTGTGGGGCCCTGGGG(+AAAAAA)GATCCTCGTTCCTGGCCTTGCCCTTCCTGGC NGG 10 8 17 8 24 0 100 5 10
    240606173422_7b3o CCCTAGCCAGGAAGGGCAAGGCCAGGAACGAG(+AAAAAA)GATCCCCCAGGGCCCCACAGTGCCTGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606173420_9ize AGAGGGCACCGGTGGGAGTCCTGGGGTTGGTG(+AAAAAA)GTACCTTCATGTGCTCAGCATCTCGCCAGCC NGG 10 8 17 8 24 0 100 5 10
    240606173419_x6pl GTCAAGGCTGGCGAGATGCTGAGCACATGAAG(+AAAAAA)GTACCACCAACCCCAGGACTCCCACCGGTGC NGG 10 8 17 8 24 0 100 5 10
    240606173417_ozdr ATGAGGCCAGGAGAGGGTACTGGATGGTACGG(+AAAAAA)GATCCCCTGGACCTGGAGATACAGATGGTTC NGG 10 8 17 8 24 0 100 5 10
    240606173414_k10x GCTTAGAACCATCTGTATCTCCAGGTCCAGGG(+AAAAAA)GATCCCGTACCATCCAGTACCCTCTCCTGGC NGG 10 8 17 8 24 0 100 5 10
    240606173413_d5km GCACCTCAGGGAGATAGTGCCTGGTGACTTTG(+AAAAAA)GATCCTGAGTTTGAAATCAGGTGTAGAGAGT NGG 10 8 17 8 24 0 100 5 10
    240606173412_0pwa ACTAGACTCTCTACACCTGATTTCAAACTCAG(+AAAAAA)GATCCAAAGTCACCAGGCACTATCTCCCTGA NGG 10 8 17 8 24 0 100 5 10
    240606173408_uwhp TAGCTCTGCTGAACCAGCAGCCCCCTCCCCAG(+AAAAAA)GATCCTCAGGAAGGAGGGGGCACATGGCTGG NGG 10 8 17 8 24 0 100 5 10