Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606173445_d8f8 GGAACTCTGACATACTCATTTGGCTTCTGTCG(+AAAAAA)GTACCAGGCACACACACGGGGCACTTACATA NGG 10 8 17 8 24 0 100 5 10
    240606173444_q5ys GGCCCATGGAGACCGGGCCATGGAGAGCCTTG(+AAAAAA)GTACCCGGATCGGTATACTCAGGCCATTGAA NGG 10 8 17 8 24 0 100 5 10
    240606173441_jsw9 GAGTGTTCAATGGCCTGAGTATACCGATCCGG(+AAAAAA)GTACCAAGGCTCTCCATGGCCCGGTCTCCAT NGG 10 8 17 8 24 0 100 5 10
    240606173439_f68d TCCCTTCCTCGGTATTGTCACCTATATGCCAG(+AAAAAA)GATCCCCTAGGACTTCCAAATTTCTTGAGAC NGG 10 8 17 8 24 0 100 5 10
    240606173437_gw50 GTCAGGTCTCAAGAAATTTGGAAGTCCTAGGG(+AAAAAA)GATCCTGGCATATAGGTGACAATACCGAGGA NGG 10 8 17 8 24 0 100 5 10
    240606173435_vmtc GGTGAGAAGGGTGTGGCTCGGATGGCATCCTG(+AAAAAA)GTACCCACAGTGAGCTGGTAGAATACTAAGT NGG 10 8 17 8 24 0 100 5 10
    240606173433_h9ya AGATTACTTAGTATTCTACCAGCTCACTGTGG(+AAAAAA)GTACCAGGATGCCATCCGAGCCACACCCTTC NGG 10 8 17 8 24 0 100 5 10
    240606173432_erep GAAAGGCCCAGTGTGGTACCAGACACACAGTG(+AAAAAA)GATCCTTCCAGAGGTGCAGGTCTGTTTATTA NGG 10 8 17 8 24 0 100 5 10
    240606173431_eaep ACCACTAATAAACAGACCTGCACCTCTGGAAG(+AAAAAA)GATCCACTGTGTGTCTGGTACCACACTGGGC NGG 10 8 17 8 24 0 100 5 10
    240606173429_z394 AGCTCTACTGGGGCCCTCTTGACCAGGAGTGG(+AAAAAA)GTACCAGGGGTCATTGCCAAAGTTTGAGGAC NGG 10 8 17 8 24 0 100 5 10