Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606163303_lk7x TGTAGCTCACATCTGTGGTGCTCAGAGCACCG(+AAAAAA)GTACCATCCACGTGAGATCTCTCCAGAACAC NGG 10 8 17 8 24 0 100 5 10
    240606163302_d1bm TCAAGGAACGTGCACCAAGAAGGCAGCTTGAG(+AAAAAA)GATCCTGACACTGAACTGAAGCGCGGGCATT NGG 10 8 17 8 24 0 100 5 10
    240606163301_d8yl AACCCAATGCCCGCGCTTCAGTTCAGTGTCAG(+AAAAAA)GATCCTCAAGCTGCCTTCTTGGTGCACGTTC NGG 10 8 17 8 24 0 100 5 10
    240606163259_kh5k GCAACACCATCTGGTCCCTGTTTACTGTCCTG(+AAAAAA)GTACCTGCTGTTACTCCAGTTTATTTATTCC NGG 10 8 17 8 24 0 100 5 10
    240606163257_nzn7 AGATGGGAATAAATAAACTGGAGTAACAGCAG(+AAAAAA)GTACCAGGACAGTAAACAGGGACCAGATGGT NGG 10 8 17 8 24 0 100 5 10
    240606163256_9o8n TTTTGAAGCTGCAAAGGATCTTTGGGGTTCTG(+AAAAAA)GATCCTCCAAGGAAGCAAGACACAGAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240606163255_018o ATACCCTCTCTCTGTGTCTTGCTTCCTTGGAG(+AAAAAA)GATCCAGAACCCCAAAGATCCTTTGCAGCTT NGG 10 8 17 8 24 0 100 5 10
    240606163253_0ft8 CTGAGCTACCATTCACAGACAGAGATACAGGG(+AAAAAA)GTACCCAGCCACAGTTACGACCAGCACCTCA NGG 10 8 17 8 24 0 100 5 10
    240606163251_l1z6 TCATCTGAGGTGCTGGTCGTAACTGTGGCTGG(+AAAAAA)GTACCCCTGTATCTCTGTCTGTGAATGGTAG NGG 10 8 17 8 24 0 100 5 10
    240606163250_bh6w AACAGTCTACGCAGGAGTACTGACCAGCGCAG(+AAAAAA)GTACCAGTCCTTGCCACAGTCGCCCATCCCC NGG 10 8 17 8 24 0 100 5 10