Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606163206_ik48 CAGTAGCTTGCTTGCTGGAGCAGAACTGATGG(+AAAAAA)GTACCTGTCTGTTCTGTTGTCCCTGCTCTCA NGG 10 8 17 8 24 0 100 5 10
    240606163204_ia26 ATCCTGGGTCCTACCCAGGCAGTGGAAATGAG(+AAAAAA)GTACCATTTTTAAAGTACTTGTGTGAAATTA NGG 10 8 17 8 24 0 100 5 10
    240606163203_cf4a AAGAGTAATTTCACACAAGTACTTTAAAAATG(+AAAAAA)GTACCTCATTTCCACTGCCTGGGTAGGACCC NGG 10 8 17 8 24 0 100 5 10
    240606163202_sw0h TGAATTTACACACCTGGATGGGAGACGTAGAG(+AAAAAA)GTACCACAACAGAGTTTCAGATTGGTGATAC NGG 10 8 17 8 24 0 100 5 10
    240606163201_2w7l TTCTGGTATCACCAATCTGAAACTCTGTTGTG(+AAAAAA)GTACCTCTACGTCTCCCATCCAGGTGTGTAA NGG 10 8 17 8 24 0 100 5 10
    240606163200_x5yz TTTCTCAATAAAAATAGGCCAGTCGCTAGAAG(+AAAAAA)GTACCATTCTGTGAAGACATTATTTTGAAGC NGG 10 8 17 8 24 0 100 5 10
    240606163158_6l3a CTAGGGCTTCAAAATAATGTCTTCACAGAATG(+AAAAAA)GTACCTTCTAGCGACTGGCCTATTTTTATTG NGG 10 8 17 8 24 0 100 5 10
    240606163157_5oxg GAGAAGGACATCCAGAGACTGTCCCACTTAGG(+AAAAAA)GATCCATTCCATCTACAGAGACCAAAACCAG NGG 10 8 17 8 24 0 100 5 10
    240606163156_1qzz AGTGTCTGGTTTTGGTCTCTGTAGATGGAATG(+AAAAAA)GATCCCTAAGTGGGACAGTCTCTGGATGTCC NGG 10 8 17 8 24 0 100 5 10
    240606163155_fo4a CCATCTTACTGCATAAAATAGCACTTTGACAG(+AAAAAA)GTACCGGCCCTCTTTATATGAAAGAAAATTT NGG 10 8 17 8 24 0 100 5 10