Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606164402_1bwq GGGCTGATAAGGGGGAGTTTATGGGATACATG(+AAAAAA)GATCCGAGGGTTGCATGACAGATTGGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240606164401_ege6 TGTTACTACATCTGGCTGCAGTAAGACTTTTG(+AAAAAA)GATCCAATGTAAACAGGAAGGGTAAGCATGT NGG 10 8 17 8 24 0 100 5 10
    240606164400_3py4 GGGGAACATGCTTACCCTTCCTGTTTACATTG(+AAAAAA)GATCCAAAAGTCTTACTGCAGCCAGATGTAG NGG 10 8 17 8 24 0 100 5 10
    240606164359_qpvt TGTATGTATGTGTGCATATCATGTGTGTTCTG(+AAAAAA)GTACCTGTGGAGATTAGGAGAGACTGTCCGG NGG 10 8 17 8 24 0 100 5 10
    240606164358_m5h6 GGGCACCGGACAGTCTCTCCTAATCTCCACAG(+AAAAAA)GTACCAGAACACACATGATATGCACACATAC NGG 10 8 17 8 24 0 100 5 10
    240606164356_g5mk AGAGGCCGGGTTTACTTTGATAGGCTAAATAG(+AAAAAA)GTACCCCAGTTAGCTGTTTGTTTTGGATTTG NGG 10 8 17 8 24 0 100 5 10
    240606164355_wjan ATTCTCAAATCCAAAACAAACAGCTAACTGGG(+AAAAAA)GTACCTATTTAGCCTATCAAAGTAAACCCGG NGG 10 8 17 8 24 0 100 5 10
    240606164353_2eys GGCCAGACAAGGCGGCCCAGTTAAGAGAACAG(+AAAAAA)GATCCACAGGCAGCTCCCTGCTCCAGTTGTT NGG 10 8 17 8 24 0 100 5 10
    240606164352_2ake TCCTCAACAACTGGAGCAGGGAGCTGCCTGTG(+AAAAAA)GATCCTGTTCTCTTAACTGGGCCGCCTTGTC NGG 10 8 17 8 24 0 100 5 10
    240606164350_hvzn TTGTTGAAGCTGCTGGCCACCAGCAATGATAG(+AAAAAA)GATCCAATGGCGAGTTGGGCCGTATCTGAGA NGG 10 8 17 8 24 0 100 5 10