Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606163219_6r1g AAATGATAGAAGTCTTTAAGGTAATTCTTTAG(+AAAAAA)GATCCTGGGAAATTCTCTCAGGTAAAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606163218_vue6 CCTCAGAAGCTATGTATTAAGCTGTGCTCGCG(+AAAAAA)GTACCTAATACAAAATACACTATATTTTGAT NGG 10 8 17 8 24 0 100 5 10
    240606163217_wo3r GGCAAATCAAAATATAGTGTATTTTGTATTAG(+AAAAAA)GTACCGCGAGCACAGCTTAATACATAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240606163215_iyys CTTTTACTTGCCTGAGTCAACATTTTTAAAGG(+AAAAAA)GATCCATTTATTCTTCCTTTGTAGTTTTCGC NGG 10 8 17 8 24 0 100 5 10
    240606163214_mxca GTAAAGCGAAAACTACAAAGGAAGAATAAATG(+AAAAAA)GATCCCTTTAAAAATGTTGACTCAGGCAAGT NGG 10 8 17 8 24 0 100 5 10
    240606163212_ihkf GCTACTGTACTTGCCTCTGCCTGAGCACCAGG(+AAAAAA)GATCCAAATCATTGTATAACACACCTCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606163211_4z26 TCTCTCCAGGAGGTGTGTTATACAATGATTTG(+AAAAAA)GATCCCTGGTGCTCAGGCAGAGGCAAGTACA NGG 10 8 17 8 24 0 100 5 10
    240606163210_b0pz CTCCTCGTGTTCTGTTAGAACTCAAGGACAAG(+AAAAAA)GTACCACAGAGAACACCTGCAATGTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606163209_8vm7 GTGACAAAGAAACATTGCAGGTGTTCTCTGTG(+AAAAAA)GTACCTTGTCCTTGAGTTCTAACAGAACACG NGG 10 8 17 8 24 0 100 5 10
    240606163207_qspb CCACCTGAGAGCAGGGACAACAGAACAGACAG(+AAAAAA)GTACCCATCAGTTCTGCTCCAGCAAGCAAGC NGG 10 8 17 8 24 0 100 5 10