Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606163153_v85n AATTTAAATTTTCTTTCATATAAAGAGGGCCG(+AAAAAA)GTACCTGTCAAAGTGCTATTTTATGCAGTAA NGG 10 8 17 8 24 0 100 5 10
    240606163152_rlwl TTGTAGTTGCCCTGAACCAATCTCTATTCTAG(+AAAAAA)GTACCGTGACACCAGTTGAAACAGACCCACC NGG 10 8 17 8 24 0 100 5 10
    240606163151_rifx AGGACGGTGGGTCTGTTTCAACTGGTGTCACG(+AAAAAA)GTACCTAGAATAGAGATTGGTTCAGGGCAAC NGG 10 8 17 8 24 0 100 5 10
    240606163150_z328 GCATTTGATGTTTGTAAATAAATCCACAGTAG(+AAAAAA)GATCCAAGCTGAAGAGGTGATACATGGTCAG NGG 10 8 17 8 24 0 100 5 10
    240606163148_xgo7 CTAGGCTGACCATGTATCACCTCTTCAGCTTG(+AAAAAA)GATCCTACTGTGGATTTATTTACAAACATCA NGG 10 8 17 8 24 0 100 5 10
    240606163147_qb50 ATCCATGATGACTTCCAAGGACTGTGTGTTAG(+AAAAAA)GTACCCGAAAAAGCTCCAAAGCTTGAATCAG NGG 10 8 17 8 24 0 100 5 10
    240606163146_vtcw ATAGTCTGATTCAAGCTTTGGAGCTTTTTCGG(+AAAAAA)GTACCTAACACACAGTCCTTGGAAGTCATCA NGG 10 8 17 8 24 0 100 5 10
    240606163144_d4gl TGCTAAGCATGATCTAGAATAGAAGCCACTGG(+AAAAAA)GTACCCCTAACTATATTTAGTGATTTAGAAC NGG 10 8 17 8 24 0 100 5 10
    240606163142_lg66 GCTGAGTTCTAAATCACTAAATATAGTTAGGG(+AAAAAA)GTACCCAGTGGCTTCTATTCTAGATCATGCT NGG 10 8 17 8 24 0 100 5 10
    240606163141_3j0k CAGTGCTCACCAGCTGTGATGTTGAAAACAAG(+AAAAAA)GATCCAAAGGTGGACATTGACGATTTGAGGT NGG 10 8 17 8 24 0 100 5 10