Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606163234_vg5g ATGTGTATGAGTGTTTGCCTGAGTATGTCTAG(+AAAAAA)GTACCATGTGCATTCAGTGCCTGTGGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240606163233_yc16 GCACATACGCTCACGTGTATCTTCTTTTGAAG(+AAAAAA)GTACCTTCTTTACCTAGCAGTTTAACACATC NGG 10 8 17 8 24 0 100 5 10
    240606163231_tn6q CTAAAGATGTGTTAAACTGCTAGGTAAAGAAG(+AAAAAA)GTACCTTCAAAAGAAGATACACGTGAGCGTA NGG 10 8 17 8 24 0 100 5 10
    240606163230_mmw0 AAATTGGGTGCATTCATTGACTCCAGAGTTAG(+AAAAAA)GTACCCAAGCATACACTAAGAAGAACCCTCT NGG 10 8 17 8 24 0 100 5 10
    240606163228_ozoc AGTGAAGAGGGTTCTTCTTAGTGTATGCTTGG(+AAAAAA)GTACCTAACTCTGGAGTCAATGAATGCACCC NGG 10 8 17 8 24 0 100 5 10
    240606163227_svw2 CAAGTGCTCTGAACTGCTGAGCATCTCTCTAG(+AAAAAA)GATCCATTGGTTTTCTTTGTTTTTTTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240606163225_rt8h TAAAAATTAAAAAAAAACAAAGAAAACCAATG(+AAAAAA)GATCCTAGAGAGATGCTCAGCAGTTCAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606163224_sa30 TCTGAGAAACCTGCTATTGACTCATTCCTTTG(+AAAAAA)GATCCTTCAACTCTAATTAAAAATGCACAGG NGG 10 8 17 8 24 0 100 5 10
    240606163222_6f92 TATTTCCTGTGCATTTTTAATTAGAGTTGAAG(+AAAAAA)GATCCAAAGGAATGAGTCAATAGCAGGTTTC NGG 10 8 17 8 24 0 100 5 10
    240606163220_unvu GTAATCTTTCTTTACCTGAGAGAATTTCCCAG(+AAAAAA)GATCCTAAAGAATTACCTTAAAGACTTCTAT NGG 10 8 17 8 24 0 100 5 10